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Introduction of the glucocorticoid binding sequences into the expression vector pΔSVE-CAT and its effect on the CAT gene expression in mammalian cells

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Sokół-Misiak W., Markiewicz W. T., Szydłowska B., Petrenko A.

Acta Biochimica Polonica

1991

vol. 38

issue 1

17-24

Mikromacierze DNA

100%

Kisiel A., Skąpska A., Markiewicz W. T., Figlerowicz M.

Kosmos

2004

vol. 53

issue 3-4

295-303

Summary The breakthrough in DNA sequencing technology and completion of sequencing of first eucaryotic genomes raised the need for high-throughput methods of gene function analysis. To solve this problem the DNA microarray technology has been developed. It is based on traditional transcript profiling methods which use the hybridization ability of DNA and RNA to monitor gene expression. Due to miniaturisation and the use of fluorescent dyes DNA microarrays allow for simultaneous monitoring of the expression of tens of thousands genes. There are two main types of DNA microarrays: cDNA microarrays and oligonucleotide microarrays, also called DNA chips. In the former, several- hundred-nucleotide long cDNA probes are printed on a glass plate and hybridized to a fluorescently labeled target cDNA obtained from the tissue of interest. In the latter, type of microarrays, each gene is represented by several short oligonucleotides (about 30 nt), perfectly matching the target gene, and several oligomers with a single mismatch. Oligonucleotides are usually synthetised on a glass slide using the photolithographic technique and the slide is hybridized to fluorescently labeled target RNA. Analysis ofmicroarray data includes the comparison of gene expression in the experimental and control probes or the comparison of gene expression profiles obtained in several experiments, by different clustering methods. Medicine is one of the fields where DNA microarrays have already found practical application. At present they are especially useful in cancer research. DNA microarray analysis of tumor tissues allows to differentiate among various cancer types, to prognose the illness progress and plan the therapy. DNA microarrays are also an irreplaceable tool in a search for new drugs.

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1 Acta Biochimica Polonica

1 Kosmos

1 2004

1 1991

2 Markiewicz W. T.

1 Figlerowicz M.

1 Kisiel A.

1 Petrenko A.

1 Skąpska A.

1 Sokół-Misiak W.

1 Szydłowska B.

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Introduction of the glucocorticoid binding sequences into the expression vector pΔSVE-CAT and its effect on the CAT gene expression in mammalian cells

Mikromacierze DNA