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1

The influence of immobilization on the overall rate of substrate biodegradation by bacterial cells

100%
Wojnowska-Baryla I.
Biotechnologia
|
1997
|
issue 3
88-99
EN
The paper includes the results of mass transfer influence on the overall rate of substrate biodegradation by bacterial cells immobilized in alginate gel. The overall rate of substrate biodegradation by immobilized bacteria is the function of the following parameters: ref = f (rmax, kl2, Deff). The results indicate that overall ratio of substrate biodegradation by immobilized bacteria is limited by external and internal transport. The effectiveness of the substrate biodegradation depends on the value of overall effectiveness factor. When limiting the overall rate of substrate biodegradation by the diffusion in a phase liquid-beads surface, the effectiveness factor is the function of Damkohler number. In the case of the internal diffusion limiting the overall rate of substrate biodegradation by immobilized bacteria the effectiveness factor depends on the Thiel number.
2

Using biosorbents in the proccesses of metal removal from water solution

64%
Wojnowska-Baryla I., Klimiuk E.
Biotechnologia
|
1997
|
issue 4
125-134
EN
This paper presents an overview of the theoretical and practical results of the heavy metals uptake by biosorbents. The influence of different factors on the heavy metal uptake by biosorbents was documented. Biosorption and related desorption mechanism were emphasized in this paper.
3

Cadmium removal by alginate beads in an air-lift reactor

64%
Wojnowska-Baryla I., Babuchowski A.
Biotechnologia
|
1997
|
issue 2
186-194
EN
This paper presents the results of a research on cadmium removal by alginate beads from an aqueous solution in an air-lift reactor. The research focused on the ability of alginate to establish an unstable coupling between cadmium and ionogenic groups of this polysaccharide. Cadmium removal by the alginate beads took place in the air-lift reactor in two phases: sorption and desorption.. The efficiency of cadmium uptake by the alginate beads in the air-lift reactor was investigated in relation to the influent concentration of cadmium amounting to 5, 10, 50 mg Cd+2? dm 3 at flow rates of 2 and 4 L ? min-1. The efficiency varied from 48.5% to 91.9% depending on the sorption duration. Cadmium recovery from the alginate beads was achieved by reducing pH up to 1.8 by 0.5 M nitric acid. The efficiency of cadmium recovery amounted to 59.8 - 96.0 %.
4

Characteristics of the ability to uptake cadmium by bacteria biosorbents

51%
Wojnowska-Baryla I., Stolarczyk E., Gredzinska A.
Biotechnologia
|
1997
|
issue 4
113-124
EN
The ability of the following bacteria: Escherichia coli, Kliebsiella pneumoniae, Proteus mirabilis, Proteus vulgaris, Pseudomonas sp. to uptake cadmium from water solution was investigated. Based on the parameters of Freundlich and Langmuir equations it was found that the kind of equation describing cadmium uptake by bacteria cells depended on the range of initial cadmium concentration in solution. Cadmium uptake by the investigated strains of bacteria from solution with low initial concentration of cadmium proceeded according to the Freundlich model. Increasing of the initial cadmium concentration above 30 mg Cd2+? dm-3 resulted in a change of the cadmium uptake mechanism and sorption was according to the Langmuir model. The best biosorbents were Pseudomonas sp. and Proteus vulgaris which showed high sorption ability in a wide range of initial concentrations (1-200 mg Cd2+ ? dm-3). In the range of low initial cadmium concentration (1-30 mg Cd2+? dm-3), the most effective biosorbent was Kliebsiella pneumoniae.
5

Rates of pollutants removal and enzyme activities in the activated sludge from anaerobic-aerobic system

51%
Stachowiak D., Klimiuk E., Wojnowska-Baryla I., Stolarczyk E.
Biotechnologia
|
1997
|
issue 3
100-108
EN
In the presented work two enzyme activity tests were performed: alkaline phosphates activity to control phosphorus removal and nitrite reductase activity to control the denitrification process. @urj
6

Nitrogen and phosphorus compounds removal by immobilized activated sludge in Sequencing Batch Reactor

51%
Wojnowska-Baryla I., Stachowiak D., Klimiuk E.
Biotechnologia
|
1997
|
issue 3
78-87
EN
Activated sludge with enhanced ability of phosphorus storage and nitrification immobilized in alginate and alginate-PVA was applied to remove phosphorus and nitrogen from waste water. The activated sludge immobilized in alginate achieved higher efficiency of phosphorus removal. It was 18% higher than in the case of activated sludge. The rates of nitrogen removal by the activated sludge and the activated sludge immobilized in alginate were similar and amounted to 0.021 and 0.190 mg TN?g-1MLSS?min-1. For the activated sludge immobilized in alginate the nitrification rate was 6 times lower than for the activated sludge. However, in the activated sludge immobilized in alginate-PVA nitrification did not occur.
7

Characteristics of the ability to uptake cadmium by free and immobilized bacteria Kliebsiella pneumoniae

51%
Wojnowska-Baryla I., Stolarczyk E., Gredzinska A.
|
EN
The ability to uptake cadmium of the following biosorbents: alginate gel, free and immobilized in alginate bacteria Kliebsiella pneumoniae was characterized. An average efficiency of cadmium removal from solution with initial cadmium concentration range from 1 to 200 mg Cd2+?dm-3 was 78,6% by alginate gel and immobilized bacteria Kliebsiella pneumoniae and 60% by free bacteria. The maximum efficiency of cadmium removal from solution with initial concentration 1 mg Cd2+? dm-3 was obtained for bacteria immobilized in alginate gel.
8

Poly-beta-hydroxybutyrate (PHB) storage and degradation in activated sludge under aerobic conditions and at high ammonium concentration in wastewater

51%
Dobrzynska A., Wojnowska-Baryla I., Bernat K.
Biotechnologia
|
2005
|
issue 1
183-193
EN
Numerous studies confirm poly-beta-hydroxybutyrate (PHB) synthesis by activated sludge under anoxic/aerobic conditions at high COD/N ratio in wastewater. In the presented experiment poly-beta-hydroxybutyrate (PHB) storage and degradation were observed in activated sludge at low COD/N ratio ? 3 in wastes. The researches were carried out in a single-stage system with activated sludge under constant oxygen supplied conditions. Readily biodegradable fraction increased in municipal wastewater through the addition of 0,2 g/dm3 acetate. Moreover, ammonium nitrogen was added to the wastes on the level of 50 mgNH4+/dm3. During the reaction time, organic carbon compounds oxidation by activated sludge and intracellular poly-beta-hydroxybutyrate (PHB) accumulation were observed. Under aerobic conditions and at low COD/N ratio ? 3 in wastewater, activated sludge used accumulated polymer as endogenous carbon source for denitrification. The obtained results show that poly-beta-hydroxybutyrate (PHB) synthesis is possible under fully aerobic conditions and at low COD/N ratio.
9

Removal of nitrogen compounds in aerobic activated sludge process

51%
Bernat K., Wojnowska-Baryla I., Dobrzynska A.
Biotechnologia
|
2005
|
issue 1
194-206
EN
In sequencing reactors without separate nitrification and denitrification zones, controlling of oxygen and organic compounds concentration influences activated sludge nitrogen removal from wastewater. Under oxygen limited conditions, autotrophic nitrifying bacteria are able to nitrate or nitrites reduction to nitrous oxide or gaseous nitrogen. However, denitrifying microorganisms can carry out heterotrophic nitrification or aerobic denitrification at high oxygen concentration. Constant amount of oxygen concentration was supplied to the sequencing batch reactor with activated sludge, which indicates that oxygen concentration could change during the reaction time. It was assumed that control oxygen supply on the level of 2 mg/dm3 covers an oxygen demand in organics, ammonium nitrogen oxidation and endogenous respiration. In the initial phase of the reaction, total oxygen exhaustion was observed. After the reaction of oxidation, there was an increase in oxygen concentration to the value of 2 mg/dm3. In the presented experiment, it was shown that permanent complete oxygen using and the accessibility of readily biodegradable organic compounds in wastewater stimulate simultaneous nitrification and denitrification in activated sludge. Nitrogen removal effectiveness under constant aeration depending on COD/N ratio in wastewater supplying to the reactor ranged from 30 to 70%.
10

Biodegradation of pollutions using an activated sludge immobilized inside the ceramic carrier

45%
Wojnowska-Baryla I., Babuchowski A., Zielinska M., Wojnowska-Bary?a I., Wojnowska-Bary?a I.
Biotechnologia
|
2001
|
issue 1
161-170
EN
Carbon and nitrogen removal in dependence on the retention time and pollution loading was investigated using biomass immobilized inside macroporous ceramic carrier. Efficiency of carbon removal was 78%, nitrification rate ? 91,7%, denitrification rate ? 64,9%, when the technological parameter were: volumetric loading 5,9 g/dm3 d, hydraulic retention time 34 min, intrinsic recirculation 50 dm3/h.
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