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1

Preliminary studies of phthalocyanine sensitizers incorporated into human leukemia cells from two cell-lines.

100%

Wiktorowicz K., Cofta J., Dudkowiak A., Waszkowiak A., Frąckowiak D.

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vol. 51

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issue 3

703-710

EN

Three phthalocyanine dyes-sensitizers were incorporated into two types of human T leukemia cells from two cell-lines: CCRF and MOLT 4. The efficiency of the dye incorporation into cells and photochemical properties of stained cells were investigated using fluorescence spectroscopy. The dyes exhibited different properties in each of the two cell-lines. Small differences in cell membrane properties have a strong influence on the efficiency of dye incorporation and on the course of photodynamic reaction. It is suggested that, for a given patient, an optimal dye-sensitizer should be established before photodynamic treatment.

2

Incorporation of stilbazolium merocyanines into resting and stimulated mononuclear leukocytes

100%

Frąckowiak D., Wiktorowicz K., Cofta J., Niedbaiska M., Latosińska M.

Acta Biochimica Polonica

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1995

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vol. 42

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issue 1

61-68

3

The interactions of phthalocyanines with stimulated and resting human peripheral blood mononuclear cells.

86%

Frąckowiak D., Waszkowiak A., Manikowski H., Ion R.-M., Cofta J., Wiktorowicz K.

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2001

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vol. 48

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issue 1

257-269

EN

The interactions of two metal-free phthalocyanines [(H2Pc) and Solar Pc (with four peripherical groups: SO2N(CH2CH2OH)2)] and of one metal substituted dye (CoPc) with resting and stimulated human peripheral blood mononuclear cells (PBMC) were compared. The absorption, fluorescence, photoacoustic and EPR spectra of both resting cells and cells stimulated by phytohaemagglutinin, incubated in dimethyl sulfoxide (DMSO) with very low or 95% water content and with or without dye addition, were measured. The fate of the light absorbed by the samples was investigated. It is known that singlet oxygen production is crucial for photodynamic action of dyes. Thermal deactivation and luminescence emission compete with this process, so investigation of these alternative paths of sensitizer deactivation provides information about photodynamic action. The incorporation of the investigated dyes into cells and the perturbation of the cell structure caused by the dyes, the incubation solvent and the activator were investigated by comparing the spectral properties of PBMC before and after stimulation and incubation. Incubation of the cells for 1 h in a solution of Solar Pc in 99.5% aqueous DMSO, resulted in an efficient dye incorporation which was highly selective. Solar Pc being introduced much more efficiently into stimulated cells than into resting cells.

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3 Acta Biochimica Polonica

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3 Cofta J.

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1 Latosińska M.

1 Manikowski H.

1 Niedbaiska M.

Preliminary studies of phthalocyanine sensitizers incorporated into human leukemia cells from two cell-lines.

Incorporation of stilbazolium merocyanines into resting and stimulated mononuclear leukocytes

The interactions of phthalocyanines with stimulated and resting human peripheral blood mononuclear cells.