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1

Soluble THF receptors

100%
Jablonska E.
Postępy Higieny i Medycyny Doświadczalnej
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1997
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vol. 51
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issue 5
567-575
EN
Soluble cytokine receptors are more general phenomena and play a wider physiological role in the regulation of the immune system than previously recognized. Soluble TNF receptors - sTNF-Rp55 and sTNF-Rp75 by ability to bind to free TNF-a might play a regulatory role in a variety of normal and abnormal reactions mediated by TNF-a. Increase of sTNF-Rs levels have been observed in the serum of patients with a different diseases. Measurement of soluble TNF receptors concentrations seems to be a significant indicator in prognostic estimation of some diseases. Additionally, bioengineered soluble receptors may prove useful as specific cytokine-inhibiting therapeutic agents.
2

iNOS expression and NO production by neutrophils in cancer patients

51%
Jablonska E., Puzewska W., Marcinczyk M., Grabowska Z.
Archivum Immunologiae et Therapiae Experimentalis
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2005
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vol. 53
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issue 2
175-179
EN
The tumor-polymorphonuclear neutrophil (PMN) relationship can be altered by the release of toxic molecules, such as nitric oxide (NO). The aim of the present study was to examine the expression of the inducible synthase of NO (iNOS) and NO production by human neutrophils of patients with oral cavity cancer. For comparison we performed similar examinations in autologous peripheral blood mononuclear cells (PBMCs). PMNs and PBMCs were isolated from the whole blood of 27 patients with squamous cell carcinoma of the oral cavity. iNOS protein expression in these cells was detected by Western blot. Total nitrite as an indicator of NO concentrations in the culture supernatants and the serum of patients was measured using a colorimetric assay. The PMNs of oral cavity cancer patients showed a significantly lower intensity of iNOS expression than those of healthy controls. The PBMCs of patients showed a more intensive expression of iNOS than the PMNs, but a lower intensity than the PBMCs of the controls. The expression of iNOS in rhIL-6 and rhIL-15-stimulated PMNs and PBMCs of patients increased in comparison with unstimulated cells. We observed lower productions of NO by PMNs and PBMCs of patients than those of the control group. The results revealed that altered iNOS expression and NO production are more characteristic of PMNs than of PBMCs of patients with oral cavity cancer. Additionally, this study provided new information about IL-6 and IL-15 activity in a tumor-bearing host.
3

Effect of IL-18 on IL-lbeta and sIL-1RII production by human neutrophils

51%
Jablonska E., Izycka A., Wawrusiewicz N.
Archivum Immunologiae et Therapiae Experimentalis
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2002
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vol. 50
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issue 2
139-142
EN
In the present study we investigated the effect of interleukin-18 (IL-18) on the production of interleukin-1beta (IL-1beta) and soluble interleukin-1 receptor II (sIL-lRII) by human neutrophils. The results obtained indicate that recombinant human interleukin-18 (rhIL-18) induces IL-l beta and, to a lesser extent, sIL-1RII production by human neutrophil isolated from peripheral blood. However, this effect was less important than lipopolysaccharide (LPS) stimulation. Additionally, our observations suggest that IL-18 can induce priming of neutrophils for IL-l beta and, to a lesser extent, sIL-lRII production by LPS--stimulated cells. The ability of IL-18 to serve as an effective modulator for IL-l betaand its regulatory protein may have significance in the inflammatory and immune reactions mediated by IL-l beta.
4

Toll-like receptors types 2 and 6 and the apoptotic process in human neutrophils

51%
Jablonska E., Marcinczyk M., Jablonski J.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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vol. 54
|
issue 2
137-142
EN
Introduction: Neutrophils (PMN) apoptosis plays an important role in limiting the last phase of inflammatory processes. It is unknown whether Toll-like receptor (TLR)2 acts independently or together with TLR6 in this process. Materials and Methods: The aim of this study was to estimate the relationship between the expressions of TLR2 and TLR6 and the apoptosis of human neutrophils in physiological conditions. We investigated the influence of recombinant human interleukin (IL)-18 and N-formyl-metionyl-leucyl-phenylalanine (fMLP) on the relationships between these receptors and neutrophil apoptosis. Results: Our results showed that after 4-h incubation, the percentage of apoptotic PMNs significantly increased compared with PMN counts before incubation. The stronger expression of TLR2 on the neutrophils suggests that this receptor contributes more significantly to the induction of PMN apoptosis than does TLR6. We also demonstrated an influence of recombinant human IL-18 (rhIL-18) on the expression of TLR6, whereas this effect was not observed in the expression of TLR2. We observed that both rhIL-18 and fMLP inhibited the apoptosis of PMNs and that rhIL-18 had a stronger effect than fMLP. Conclusions: The obtained results suggest that not only TLR2, but also TLR6 plays an important role in the regulation of the apoptosis of PMNs. Changes in the expression of TLR6 and inhibition of apoptosis of PMNs by rhIL-18 seem to confirm the vital role this receptor and of rhIL-18 in regulating the survival of these cells. These data can be useful in developing methods to regulate PMN apoptosis in conditions associated with their excessive and unfavorable activation.
5

Role of the p38 MAPK pathway in induction of iNOS expression in human leukocytes

51%
Jablonska E., Ratajczak W., Jablonski J.
Folia Biologica
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2008
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vol. 56
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issue 1-2
83-89
EN
Inducible nitric oxide synthase (iNOS) is one of the enzymes responsible for NO production in neutrophils (PMN) and in peripheral blood mononuclear cells (PBMC). Several studies have demonstrated that iNOS expression is controlled by a wide group of cytokines which achieve their biological effect through, among others, the activation of the p38 MAPK pathway. The aim of the present study was to define the participation of the p38 MAPK pathway in the induction of iNOS expression and NO production by PMN and PBMC of healthy persons after stimulation of rhIL-15 and rhIL-18. We also estimated the influence of rhIL-15 and rhIL-18 on cGMP production by both population cells and the production of superoxide anion radicals by neutrophils. The results show that rhIL-15 and rhIL-18 induced an increase in the expression of iNOS and phospho-p38 MAPK in PMN and PBMC. We also found that PMN and PBMC, stimulated by these cytokines, released larger amounts of NO and cGMP in comparison with non-stimulated cells. Additionally, PMN showed a more pronounced ability to produce superoxide anions. The results suggest that iNOS activation in neutrophils and in peripheral blood mononuclear cells stimulated with rhIL-15 and rhIL-18 may be achieved through the assistance of the p38 MAPK pathway.
6

Interleukin 6, tumor necrosis factor alpha and their soluble receptors in the blood serum of patients with denture stomatitis and fungal infection

39%
Pietruski J. K., Pietruska M. D., Jablonska E., Sacha P., Zaremba M., Stokowska W.
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|
vol. 48
|
issue 2
101-105
EN
Determinations of the blood serum levels of interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha) and their soluble receptors (sIL-6R, sTNFR) in denture stomatitis patients (DS) were performed. Serum levels of interleukins and their soluble receptors were measured using the ELISA method. In all examined patients mycological diagnostics were conducted using API 20C AUX stripe tests and an automatic ATB machine. Results were compared with those of healthy denture weares (D), and controls (C). In DS patients, yeasts were isolated in 90. 9%, in D in 66. 7% of cases. The most often isolated species in both groups was Candida albicans. Mean concentrations of IL-6 and TNF-alpha were statistically significantly higher in D and D groups compared to controls. Mean concentrations of sIL-6R were similar in all groups; however, concentrations of sTNFR in both DS and D groups were significantly lower compared to controls. There were no correlations found between values of IL-6 and TNF-alpha nor between examined interleukins and their soluble receptors.
7

TNF-alpha, IL-6 and their soluble receptor serum levels and secretion by neutrophils from cancer patients

39%
Jablonska E., Kiluk M., Markiewicz W., Piotrowski L., Grabowska Z., Jablonski J.
Archivum Immunologiae et Therapiae Experimentalis
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2001
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vol. 49
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issue 1
63-70
EN
Simultaneous evaluation of cytokines and their soluble receptor production and serum levels can be helpful in understanding the local and systemic immune response of a tumor-bearing host. In the present study we examined the serum levels of TNF-, IL-6 and their soluble receptors sTNFRp55, sTNFRp75 and sIL-6R compareded with their production by the polymorphonuclear neutrophils (PMN) from cancer patients. Examinations were carried out in patients with adenocarcinoma breast cancer and squamous cell carcinoma of the oral cavity and related to the clinical course and to different phases of therapy. Secretion of IL-6, sTNFRp55 and sTNFRp75 by PMN appeared to be dependent on tumor type, clinical progression of disease as well as on therapy, suggesting a significant role of these cells at different phases of the immune response to cancer associated with these mediators. Changes in values of TNF-alpha, IL-6 and their soluble receptors in sera of both cancer groups, dependent on tumor type, clinical progression and cancer therapy, could have a diagnostic and prognostic role in cancer disease.
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