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Usefulness of real-time PCR in long-term follow-up of follicular lymphoma patients

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Tysarowski A., Fabisiewicz A., Paszkiewicz-Kozik E., Kulik J., Walewski J., Siedlecki J. A.

2007

vol. 54

issue 1

135-142

The aim of this study was to evaluate the usefulness of quantitative real-time PCR (RQ-PCR) for the monitoring of molecular remission in follicular lymphoma (FL) patients during long-term follow-up. RQ-PCR by the use of TaqMan® detection system is a sensitive tool to monitor minimal residual disease (MRD) in FL through amplification of the t(14;18) fusion gene during and post-therapy. In most cases the breakpoint region occurs within the major breakpoint region (MBR). Among 75 patients diagnosed with FL, cells harboring the fusion gene BCL2/JH were found in peripheral blood of 31 patients (41%). We further monitored 30 of these patients in a period varying from 6 months to 5 years by RQ-PCR. In our study the level indicating the possibility of the presence of MRD was established at more than five t(14;18)-positive cells in the background of 83000 normal cells. The results of this work also confirmed that the presence of MRD detected by RQ-PCR is an indication for careful observation of patients because of a higher risk of disease recurrence.

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1 Acta Biochimica Polonica

1 2007

1 Fabisiewicz A.

1 Kulik J.

1 Paszkiewicz-Kozik E.

1 Siedlecki J. A.

1 Tysarowski A.

1 Walewski J.

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Usefulness of real-time PCR in long-term follow-up of follicular lymphoma patients