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Pulse-polarographic characteristic of DNA modified by organophosphorus insecticides in vitro

100%
Wiaderkiewicz R., Walter Z., University of Łódź D. o. M. G.
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
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1990
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vol. 7
PL
Przeprowadzono badania metodą puls-polarograficzną DNA izolowanego z grasicy cielęcia i zmodyfikowanego związkami fosforoorganicznymi. Zbadano wpływ następujących związków: malationu (0,0-dimetylo-S-1-2-dikarboetoksyetyloditiofosforanu), DDVP (0,0-dimetylo-0-1-2-dichlorowinylofosforanu), metyloparationu (0,0-dimetylo-0-4-nitrofenylotionofosforanu) i metylobromfenwinfosu (0,0-dimetylo- 0-1-2,4-dichlorofenylo-2-bromowinylofosforanu). Zaobserwowano zmiany własności pulspolarograficznych po inkubacji dwuniciowego DNA ze związkami fosforoorganicznymi. Zmiany dotyczyły szczytu II i III. Szczyt II jest charakterystyczny dla dwuniciowego DNA i jest wywołany niewielkimi zaburzeniami helikalnej struktury. Szczyt III jest charakterystyczny dla jednoniciowego DNA. Po 72-godzinnej inkubacji z malationem, metyloparationem lub metylobromfenwinfosem szczyt III odpowiadał 2-3% zawartości jednoniciowego DNA w odniesieniu do zdenaturowanego termicznie DNA jako wzorca. Pomiary w pH 5 wykazały różnice w kinetyce zmian dwuniciowego DNA między DDVP i innymi badanymi insektycydami. Zmiany strukturalne DNA wywołane przez malation, metyloparation i metylobromfenwinfos były bardzo nieznaczne. Obserwowano zmiany w zakresie temperatur przedtopnieniowych (30-60°c). Polarograficzne pomiary przeprowadzone po termicznej denaturacji dwuniciowego DNA, zmodyfikowanego uprzednio związkami fosforoorganicznymi, wykazywały obecność regionów termolabilnych.
2
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Expression of cytochrome CYP2B1/2 in nonpregnant, pregnant and fetal rats exposed to tobacco smoke.

81%
Czekaj P., Wiaderkiewicz A., Florek E., Wiaderkiewicz R.
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2000
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vol. 47
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issue 4
1115-1127
EN
Four-month-old female Wistar rats were exposed for 20 days to tobacco smoke obtained from non-filter cigarettes. During the exposure, concentration of tobacco smoke was monitored indirectly by measuring the CO level (1500 mg/m3 air). The efficacy of exposure was assessed by measuring urine nicotine and cotinine levels. Cigarette smoke did not change total cytochrome P450 and b5 protein levels in any of the organs studied, and most of these organs did not show any changes in the activity of reductases associated with these cytochromes. Following exposure to tobacco smoke, fetal rat liver expressed CYP2B1/2 protein; in newborns (day 1) both liver and lung showed CYP2B1/2 protein expression and very low pentoxyresorufin O-dealkylase activity. Western blot analysis of adult liver, lung, heart, but not of brain microsomes, showed that tobacco smoke induced CYP2B1/2 in both nonpregnant and pregnant rats, though its expression was lower in the livers and hearts of pregnant females. In the rat and human placenta, neither rat CYP2B1/2 nor human CYP2B6 showed basal or tobacco smoke-induced expression at the protein level. This study shows clearly that the expression of CYP2B1/2, which metabolizes nicotine and some drugs and activates carcinogens, is controlled in rats by age-, pregnancy-, and tissue-specific regulatory mechanisms.
3
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Estimation of the extent of platination and the sites of Pt binding after interaction of cis- and trans- diamminedichloroplatinum(II) with DNA and chromatin

81%
Oliński R., Wiaderkiewicz R., Walter Z.
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vol. 32
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issue 2
119-125
4
Content available remote

Sites of methylation of DNA bases by the action of organophosphorus insecticides in vitro

80%
Wiaderkiewicz R., Walter Z., Reimschussel W.
Acta Biochimica Polonica
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1986
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vol. 33
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issue 2
73-85
5
Content available remote

Arterial ketone index in assessing liver function and its detoxicative capability after ischemia-reperfusion injury.

71%
Caban A., Wiaderkiewicz R., Kamiński M., Oczkowicz G., Ziaja J.
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2000
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vol. 47
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issue 4
1137-1146
EN
Arterial ketone index (AKBR) which is the ratio of acetoacetic acid to 3-hydroxybutyric acid in the arterial blood, is believed to reflect the mitochondrial reduction potential of hepatocytes and general energy state of the liver. In the presented paper we challenged this hypothesis by analysing the correlation between AKBR and the results of typical liver blood tests (AspAT, AlAT, LDH, CRP) and biotransforming potential of the liver (cytochromes P450, b5 and their corresponding NADPH and NADH reductases) in the model of ischemia-reperfusion injury of rat liver. The results were compared with histochemical analysis of distribution and activity of SDH, LDH and G-6-Pase, the key marker enzymes of the liver. We have shown that, except in the case of acute phase protein (CRP), a decrease in AKBR correlated well with the increase of the level of indicator enzymes in serum. Histochemical analysis also confirmed that AKBR correlates with the degree of damage to hepatocytes during early stage of reperfusion after 60 min of liver ischemia. In the Spearman test, AKBR was significantly correlated with the changes in cytochrome P450 content and its NADPH reductase activity which indicates a high sensitivity of this test. We conclude that the decrease of AKBR value reflects the impairment of basic energy pathways and detoxicative capability of the liver.
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