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Dietary intake of specific phenolic compounds and their effect on the antioxidant activity of daily food rations

100%
Koch W., Baj T., Kukula-Koch W., Marzec Z.
Open Chemistry
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2015
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vol. 13
|
issue 1
EN
The determination of phenolic acids’ and flavonoids’ content in daily food rations (DFRs) of a selected group of male and female students and the development of chromatographic conditions is the primary goal of the study. The presence of 7 phenolic components were confirmed in the prepared extracts from all diets reconstructed within a period of 3 years. The highest concentrations were determined for hesperidin (124 and 55.6 mg for women and men, respectively), naringin (47.6 mg in female and 37 mg in male diets) and chlorogenic acid (19.7 and 19.8 mg for women and men). The antioxidant potential of the daily food rations, measured with a DPPH test, was higher for women (range 47.1‒78.8%) than for men (range 34.5‒78.0%) and was found to strongly correlate with the total phenolics content of the samples (Folin-Ciocalteu test) (correlation coefficient 0.90).
2
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Influence of extraction methods on the recovery of astragaloside IV from the roots ofAstragalus mongholicusin Soxhlet- and Twisselmann-type apparatus

64%
Kukuła-Koch W., Baj T., Sieniawska E., Koch W., Widelski J., Luchowska K., Kołodziej B., Głowniak K.
Open Chemistry
|
2015
|
vol. 13
|
issue 1
EN
Astragalus mongholicus, commonly used in Traditional Chinese Medicine (TCM), contains significant amounts of triterpene saponins – astragalosides, which possess a wide variety of pharmacological actions, including the treatment of cardiovascular and respiratory diseases. The present study was performed to develop a fast and effective extraction method of saponins from crude plant material using Twisselmann-type apparatus. Moreover, a detailed comparison between Soxhlet and Twisselmann apparatus was performed to explain the advantages and disadvantages of their applications. The content of astragaloside IV – the major saponin of A. mongholicus extracts and chosen as an extraction efficiency marker ‒ was determined by means of RP-HPLCELSD chromatography in an herein optimized method. Twisselmann extraction shortened its duration from 4 to 2 hours in comparison with the Soxhlet method ‒ a generally accepted method preparation for this extract. The effectiveness of both apparatus was alike (3.24 ± 0.44 mg and 3.48 ± 0.26 mg of astragaloside IV/ 4g of powdered root for 2 hours in Twisselmann and 4 hours in Soxhlet apparatus). Compared with a pharmacopoeial method, the application of Twisselmann apparatus enabled a decrease in analysis time of 50%, which is of high significance, especially for large, scaled-up industrial scale.
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