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Effects of dietary crude protein on slaughter yield of selected broiler stocks

100%

Melo J. E., Miquel M. C., Mallo G., Ciacciariello M., Villar E.

Journal of Applied Genetics

1999

vol. 40

issue 3

219-231

The objective of this paper was to determine the differences in yield and body composition between a commercial strain (COM) and a weakly selected strain (FR) of broilers at two slaughter ages on diets differing in protein content. There were 306 males reared on litter floor pens, fed ad libitum, and randomly assigned to 16 pens in a 2 x 2 factorial design with 4 repetitions. The diets were HP (high protein): 20.5% protein to 6 weeks of age and 16.9% from then on; LP (low protein): 16.9% protein all the time. At 50 and 71 days, four broilers from each pen were taken at random, fasted, killed, slaughtered and the following weights were recorded: live weight, eviscerated carcass, abdominal fat, feathers, blood, small intestine, large intestine, gizzard, proventriculus, liver, breast, thighs and heart. Analyses of variance for traits and for their proportions to live weight were done. The model included genotype, diet, genotype x diet and replicate. The genotypes differed in live weight and growth patterns, COM showing a higher proportion of commercial cut weight and FR a higher digestive organ and relative feather weight at older ages. The low protein diet affected COM genotypes more than FR genotypes, probably because there was a difference in protein requirements.

New polymorphism of FASN gene in chicken

73%

Marrube G., Rozen F., Pinto G. B., Pacienza N., Melo J. E., Huguet M. J., Canet Z., Zandomeni R., Miquel M. C.

Journal of Applied Genetics

2004

vol. 45

issue 4

453-455

Sequencing, Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) were carried out to detect polymorphism in the last intron of the FASN gene of the Campero broiler line. The analysis of the sequences presents a G to A substitution located at base 459 of the PCR product (GenBank accession number J02839, located at base Nr. 1222), resulting in a site recognized by restriction enzymes Hae III and Ava II. Also, the sequence presents a G to A substitution (located at base 603 of the PCR product and Nr. 1366 of the J02839 GenBank accession) resulting in a site recognized by restriction enzyme Pst I. Alleles and genotype frequencies were calculated for endonucleases Hae III, Ava II and Pst I for 44 broilers.

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2 Journal of Applied Genetics

1 2004

1 1999

2 Melo J. E.

2 Miquel M. C.

1 Canet Z.

1 Ciacciariello M.

1 Huguet M. J.

1 Mallo G.

1 Marrube G.

1 Pacienza N.

1 Pinto G. B.

1 Rozen F.

1 Villar E.

1 Zandomeni R.

Search results

Effects of dietary crude protein on slaughter yield of selected broiler stocks

New polymorphism of FASN gene in chicken