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Indocyanine green as a prospective sensitizer for photodynamic therapy of melanomas.

100%

Urbanska K., Romanowska-Dixon B., Matuszak Z., Oszajca J., Nowak-Sliwinska P., Stochel G.

2002

vol. 49

issue 2

387-391

Spectroscopic, photochemical and biological properties of indocyanine green (ICG) are presented. Light over 800 nm is effectively absorbed by ICG. This property as well as photochemical behaviour of ICG make it a very suitable dye for photodynamic treatment of melanoma cells. Cytotoxicity of ICG itself and the effect of photodynamic therapy (PDT) were evaluated by following the growth of human (SKMEL 188) and mouse (S91) melanoma cells. The surviving fraction of the cells irradiated (λex = 830 nm) vs non-irradiated, treated with the same dose of ICG, is significantly decreased (5- to 10-fold). These results show that ICG is a very promising dye for photodynamic therapy of melanomas.

Pulmonary metastases of the A549-derived lung adenocarcinoma tumors growing in nude mice. A multiple case study

84%

Jakubowska M., Śniegocka M., Podgórska E., Michalczyk-Wetula D., Urbanska K., Susz A., Fiedor L., Pyka J., Płonka P.

2013

vol. 60

issue 3

323-330

Lung adenocarcinoma is a leading human malignancy with fatal prognosis. Ninety percent of the deaths, however, are caused by metastases. The model of subcutaneous tumor xenograft in nude mice was adopted to study the growth of control and photodynamically treated tumors derived from the human A549 lung adenocarcinoma cell line. As a side-result of the primary studies, observations on the metastasis of these tumors to the murine lungs were collected, and reported in the present paper. The metastasizing primary tumors were drained by a prominent number of lymphatic vessels. The metastatic tissue revealed the morphology of well-differentiated or trans-differentiated adenocarcinoma. Further histological and histochemical analyses demonstrated the presence of golden-brown granules in the metastatic tissue, similar to these found in the tumor tissue. In contrast to the primary tumors, the electron paramagnetic resonance spectroscopy revealed no nitric oxide - hemoglobin complexes (a source of intense paramagnetic signals), in the metastases. No metastases were found in other murine organs; however, white infarctions were identified in a single liver. Taken together, the A549-derived tumors growing subcutaneously in nude mice can metastasize and grow on site in the pulmonary tissue. Thus, they can represent an alternative for the model of induced metastatic nodule formation, following intravenous administration of the cancerous cells.

Acute hepatologic and nephrologic effects of calcitriol in Syrian golden hamster (Mesocricetus auratus)

68%

Podgorska E., Sniegocka M., Mycinska M., Trybus W., Trybus E., Kopacz-Bednarska A., Wiechec O., Krzykawska-Serda M., Elas M., Krol T., Urbanska K., Slominski A.

2018

vol. 65

issue 3

351-358

Although vitamin D is included in the group of fat-soluble vitamins, it must be considered as a prohormone. Its active forms, including calcitriol, have pleiotropic effects and play an important role in the regulation of cell proliferation, differentiation and apoptosis, as well as in hormone secretion, and they demonstrate anti-cancer properties. Since calcitriol delivery can be beneficial for the organism, and Syrian golden hamsters represent a unique experimental model, we decided to investigate its toxicity in this species. In this study, we injected calcitriol intraperitoneally at doses 0 (control), 0.180±0.009 µg/kg and 0.717±0.032 µg/kg. Animal behavior was observed for 72 hrs after injection, and afterwards blood, liver and kidneys were collected for post-mortem examination, electron microscopy, and hematology analyses. The highest dose of calcitriol induced a change in animal behavior from calm to aggressive, and the liver surface showed morphological signs of damage. Following injection of calcitriol, ultrastructural changes were also observed in the liver and kidneys, e.g. vacuolization and increased number of mitochondria. There was also a trend for increased serum levels of aspartate aminotransferase (AST), but not of alanine aminotransferase (ALT) or GGTP (gamma-glutamyl transpeptidase). There was no change in Ca, Mg and P levels, as well as in blood morphology between experimental and control groups. These results indicate that calcitriol at 0.717, but not at 0.180 µg/kg, may induce acute damage to the liver and kidneys, without inducing calcemia. We propose that the hepatotoxic effect of calcitriol in hamster constitutes the primary cause of behavioral changes.

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3 Acta Biochimica Polonica

1 2018

1 2013

1 2002

3 Urbanska K.

1 Elas M.

1 Fiedor L.

1 Jakubowska M.

1 Kopacz-Bednarska A.

1 Krol T.

1 Krzykawska-Serda M.

1 Matuszak Z.

1 Michalczyk-Wetula D.

1 Mycinska M.

1 Nowak-Sliwinska P.

1 Oszajca J.

1 Podgorska E.

1 Podgórska E.

1 Pyka J.

1 Płonka P.

1 Romanowska-Dixon B.

1 Slominski A.

1 Sniegocka M.

1 Stochel G.

1 Susz A.

1 Trybus E.

1 Trybus W.

1 Wiechec O.

1 Śniegocka M.

Search results

Indocyanine green as a prospective sensitizer for photodynamic therapy of melanomas.

Pulmonary metastases of the A549-derived lung adenocarcinoma tumors growing in nude mice. A multiple case study

Acute hepatologic and nephrologic effects of calcitriol in Syrian golden hamster (Mesocricetus auratus)