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1

Inheritance of resistance to powdery mildew (Oidium lycopersicum Cooke & Massee, emend. Noordeloos & Loerakker) in accessions of three wild species of Lycopersicon

100%
Kozik E. U.
Journal of Applied Genetics
|
1999
|
vol. 40
|
issue 3
175-183
EN
Three resistant accessions: L. hirsutum LA1775, L. pennellii LA716, L. chilense LA2747, and the susceptible breeding line A100, were crossed to develop F1, F2 and BC1 populations for genetic analysis of tomato resistance to Oidium lycopersicum Cooke & Massee, emend. Noordeloos & Loerakker, the causal agent of powdery mildew. The resistances in all the studied wild species of Lycopersicon were dominant, but controlled by different numbers of genes depending on the source of resistance. Two incompletely dominant genes control the resistance in L. hirsutum, but one major gene has a stronger expression than the second minor gene. L. pennellii carries three cumulative dominant genes. The resistance of L. chilense is governed by one partially dominant gene that is less effective than the resistance genes of L. hirsutum.
2

Towards the introducing of resistance to powdery mildew from Lycopersicon hirsutum into L. esculentum

100%
Kozik E. U.
Journal of Applied Genetics
|
2001
|
vol. 42
|
issue 1
33-42
EN
Genes of resistance to Oidium lycopersicum from Lycopersicon hirsutum LA 1775 were introduced to L. esculentum. Breeding procedures were based on a one-way programme up to the F2 generation and then four different methods were adopted to obtain F4 and BC4 populations. Screening tests among those hybrid populations were performed in a greenhouse and showed segregation for resistance to powdery mildew due to different genetic backgrounds of the families derived from four breeding methods that changed the status of the gene/genes responsible for resistance to powdery mildew. F4 and BC4 populations varied in relation to morphological traits (fruit size and weight, seed and fruit productivity, number of locules). There was a significant progress in breeding in comparison to L. hirsutum regarding fruit size and weight, and the number of locules. Values of two other traits: seed and fruit productivity, that are correlated with self- and cross-compatibility, were low and similar to L. hirsutum. Therefore, another one or two backcrosses will probably improve seed and fruit productivity.
3

Evaluation of genetic diversity and uniformity of head cabbage DH lines by the use of RAPD markers

51%
Kaminski P., Staniaszek M., Kozik E. U.
Journal of Applied Genetics
|
2003
|
vol. 44
|
issue 2
157-163
EN
DH lines derived from cabbage cvs. Kamienna Glowa, Slawa z Enkhuizen and Langendijker, representing R1 generation, were analysed by the use of RAPD markers for their diversity and uniformity. For the evaluation of genetic diversity, eight primers yielding informative bands were used. Of the total of 83 RAPD bands scored in this study, 16.9% were polymorphic between a set of 13 DH lines. The similarity of the DH lines, estimated by Jaccard?s coefficient, was depicted in the UPGMA dendrogram. Fourteen generated informative RAPD bands allowed the identification of DH lines developed from each cultivar. The evaluation of the uniformity for six closely related DH lines was possible by the use of three primers which generate one or two polymorphic bands. The lack of differences among ten plants of the five investigated DH lines manifested their uniformity. One line showed intraline polymorphism with two RAPD primers. The occurrence of the differences at the molecular level among ten plants indicated that their parental R0 plant was probably obtained from somatic cells, not by androgenesis.
4

Morphological diversity of androgenic carrot plants

39%
Kozik E. U., Nowak R., Klosinska U., Gorecka K., Krzyzanowska D., Gorecki R.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 1
49-53
EN
The aim of this study was to evaluate phenotypic variation of R0 androgenic plants obtained from four seed sources and donor plants by anther culture. Several morphological traits (leaf size, petiole length, leaf division, cortex colour) and the range of diversity were evaluated. There was large variation in all traits among the donor varieties. Especially leaf division and cortex colour differed significantly among the androgenic plants that came from different seed sources. The plants regenerated from four donor plants of variety 62 were significantly different in most traits except for leaf width and cortex colour. Evaluation of R1 plants will demonstrate whether the R0 variation observed is due to genetic variation or physiological differences from tissue culture.
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