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1

Estimation of induced mutation rates of four esterase genes in barley (Hordeum vulgare L.)

100%
Kucharska M., Maluszynski M.
Journal of Applied Genetics
|
1998
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vol. 39
|
issue 2
141-145
EN
. Induced mutation rate of barley esterase loci has been estimated. Results suggested that about 3% of investigated M1 spikes had seeds which gave rise to M2 seedlings mutated in one of four esterase loci. M1 plants were obtained after chemical treatment of seeds from two spring barley cultivars Aramir and Bielik. The majority of mutants were reconfirmed in the M3 generation.
2

Induced isozyme polymorphism in spring barley mutants

100%
Kucharska M., Maluszynski M.
Journal of Applied Genetics
|
1996
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vol. 37
|
issue 1
109
EN
The usefulness of mutagenic treatment to enlarge isozymic variability of barley and the use of induced mutants for genetic analysis were evaluated.N-methyl-N-nitroso urea, sodium azide and gamma rays were employed as mutagenic agents>Electrophoretic assays of 3848 M2 seedlings obtained by chemical mutagenic treatment of the spring barley cultivars Dema, Aramir, Bielik and 3100 M2 seedlings obtained by physical mutagenic treatment of the cv. Dema revealed 70 isozymic mutants, which represent 30 separate mutants in 25 M1 plants.Most of mutations (27) were induced by chemical mutagen at polymorphic esterase loci.The occurence of induced mutants analysis of those loci in barley including mapping respective genes within chromosomes.
3

Gene segregation in a barley DH population

81%
Szarejko I., Maluszynski M., Polok K., Zbieszczyk J.
|
|
vol. 38
|
issue 4
415-424
EN
A large population of anther culture-derived barley regenerants and their progeny was tested for allele segregation at 1 isozyme and 8 morphological marker loci. The segregation of genetic markers was examined separately for haploid, diploid and polyploid regenerants. All the 9 analysed genes except al (albino lemma) on chromosome 3 segregated according to the expected 1 : 1 ratio in the microspore-derived barley population. There was no difference in allele distribution between haploid and diploid regenerants. Among the limited number of 34 analysed tetraploids a significant excess of the dominant allele at locus o (orange lemma) of chromosome 6 was also observed. The recombination frequency between linked genes (n - lk2 on chromosome 1 and r - s on chromosome 7) estimated in the DH population did not differ significantly from recombination rates calculated in F2 progeny or presented in barley chromosome maps. The phenomenon of gametic selection is discussed in relation to the genotype dependency of anther culture response and procedures used for DH production in barley.
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