Search results

Sort By:

Limit search:

Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)

100%

Sousa M. A., Santos B. N., Carvalhal C., Steindel M.

Acta Protozoologica

2017

vol. 56

issue 2

119-127

Two KP1(–) strains of Trypanosoma rangeli (SC-58, SC-61) isolated from the wild rodent Phyllomys dasythrix from Santa Catarina (Brazil) were compared with some KP1(+) reference stocks from different Latin America countries, and also with Trypanosoma lewisi. The strains were analyzed by some morphological and biological features, and by biochemical and molecular techniques. The mean total length (TL) of the bloodstream trypomastigotes of T. rangeli varied between 31.3–33.0 µm, and those of T. lewisi (adult forms) was 28.2 µm, values within the variation known for each species. In T. rangeli KP1(+) and T. lewisi, the nucleus was located in the anterior portion of the body, with nuclear indexes (NI) ≥ 1.2, as typically described for both species. Differently, most trypomastigotes of the KP1(–) stocks presented NI ≤ 1.0. Another striking feature of the KP1(–) strains was their very fastidiously growth in axenic cultures when compared with the KP1(+) stocks and T. lewisi. Three isoenzyme loci (MDH, IDH and PGM) clearly distinguished T. rangeli and T. lewisi, and the distinction between the KP1(+) and KP1(–) strains was possible at MDH, PGM and GPI loci. All T. rangeli strains presented the typical 760 bp amplicon derived from their KP2 minicircles. However, the KP3 products of the KP1(+) strains were a single large band (~330bp), whereas those of the KP1(–) had two distinct bands (350 and 300 bp). T. lewisi presented 700 and 400 bp amplicons, as previously reported. The peculiarities of T. rangeli isolates from P. dasythrix corroborate a possible speciation process within this taxon.

Cluster Analysis of Non-conserved Proteins of Trypanosoma cruzi Reference Strains displays Parity between these Groupings (Peptidemes) and the Consensually Accepted Parasite Lineages

100%

Coelho F. S., Vieira D. P., Lopes A. H., Sousa M. A.

Acta Protozoologica

2019

vol. 58

issue 2

81-88

The protein profiles of the epimastigote stages from eight reference strains of Trypanosoma cruzi belonging to three different lineages (TcI, TcII and TcVI) were analyzed by SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis), under standardized conditions. More than 40 protein bands were observed in each strain. Around 55% of them were not shared by all stocks (non-conserved proteins), representing their intra-specific variability. Then, they were coded for processing by numerical taxonomy, using three association coefficients and the UPGMA clustering algorithm. With all coefficients assayed, two major groups were clearly seen, confirming the dichotomy within T. cruzi taxon, as demonstrated by other molecular and biochemical approaches. In the present study, the term peptideme was used to name the groups of strains based on their polypeptide profiles, following the above-cited methodology. Then, two major peptidemes were identified, each one presenting subdivisions. The isolates identified as TcI clustered in the same major peptideme, displaying a subgroup with the opossum isolates (G, SC28, Dm28c) apart from the stock of human origin (Colombian strain). The other major peptideme also showed two subgroups, regardless the coefficient used. One of them included the TcII strains (Y, SF21), both from Brazilian patients, and the other the TcVI stocks, both originally from triatomines from Southern Brazil (CL Brener, FL). As far we know, this is the first report on the parity between the T. cruzi lineages consensually accepted and their grouping into peptidemes based on SDS-PAGE and the numerical analysis of non-conserved proteins.

Refine search results

2 Acta Protozoologica

1 2019

1 2017

2 Sousa M. A.

1 Carvalhal C.

1 Coelho F. S.

1 Lopes A. H.

1 Santos B. N.

1 Steindel M.

1 Vieira D. P.

Search results

Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)

Cluster Analysis of Non-conserved Proteins of Trypanosoma cruzi Reference Strains displays Parity between these Groupings (Peptidemes) and the Consensually Accepted Parasite Lineages