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Laser- and Light-Induced Autofluorescence Spectroscopy of Human Skin in Dependence on Excitation Wavelengths

100%
Bliznakova I., Borisova E., Avramov L.
Acta Physica Polonica A
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2007
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vol. 112
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issue 5
1131-1136
EN
Human skin contains various types of native fluorophores and absorbers with unique absorption and emission spectra, different quantum efficiency, concentration and spatial distribution within the skin. Autofluorescence spectroscopy is applied as diagnostic tool for cutaneous tumor detection that increases the importance of evaluation of natural existing fluorophores and unification of data for given class of pathologies. In the current study, several excitation sources in the region 337-405 nm are applied, to achieve information about typical autofluorescent properties of normal human skin.
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Development of Low-Cost Photodynamic Therapy Device

88%
Momchilov N., Bliznakova I., Borisova E., Troyanova P., Avramov L.
Acta Physica Polonica A
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2007
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vol. 112
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issue 5
1125-1130
EN
Photodiagnosis and photodynamic therapy of non-melanoma skin cancers using delta-aminolevulinic acid/protoporphyrin IX (5-ALA/PpIX) give a combined application with broadest dissemination in the current clinical practice. The problems with using of lasers as light sources are the expenses associated with the operation of these types of installations. This is why we test the capability of cheaper sources - light-emitting diodes at 405 nm for fluorescence excitation of PpIX and 635 nm for photodynamic action initiation. A LED matrix is developed in our laboratory using two types of LEDs and a combined photodiagnosis/photodynamic theory device applicable for clinical practice is built. Geometrically matrix is formed in such way that power density at 635 nm is about 40 mW/cm^2, which allow to reach treatment doses for a 15-20 min irradiation depending of the lesion size in the focus of the system. The therapeutic mode of system developed can be used also with some other photosensitizers from the porphyrins derivatives family.
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