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1

SSCP polymorphism within 5' region of bovine lactoglobulin (LGB) gene

100%
Kaminski S., Zabolewicz T.
Journal of Applied Genetics
|
1998
|
vol. 39
|
issue 1
97-102
EN
In the paper the detection of the SSCP polymorphism within the 5' fragment of bovine beta-lactoglobulin (LGB) gene is described. The 5' fragment of LGB gene (209 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 124 animals (50 cows and 74 bulls) six SSCP patterns were identified and named R1, R2, R3, R4, R5 and R6, which occured with the frequency of 0.32, 0.51, 0.09, 0.06, 0.01and 0.01, respectively. The PCR-SSCP method is simple, fast, and relatively inexpensive. The SSCP polymorphism reported in the paper may be useful in looking for the associations between different SSCP patterns and LGB gene expression and milk properties.
2

Associations between bovine beta-lactoglobulin polymorphism within coding and regulatory sequences and milk performance traits

100%
Kaminski S., Zabolewicz T.
Journal of Applied Genetics
|
2000
|
vol. 41
|
issue 2
91-99
EN
The bovine beta-lactoglobulin (LGB) gene is considered a potential quantitative trait locus in dairy cattle breeding. In Black-and-White dairy cattle the LGB gene has two predominant alleles A and B. This can result in three possible genotypes AA, AB and BB. Moreover, within the promoter of the gene several point mutations were found. A herd of one hundred and twenty-four Black-and-White cows were genotyped for two loci: locus LGB (exon IV, alleles A and B) and locus LGB-R (SSCP polymorphism within a fragment of LGB promoter: SSCP patterns R2, R3, R1, R9). In our sample 13 AA, 58 AB and 53 BB LGB cows and 66 R2, 16 R3, 40 R1 and 2 R9 LGB-R cows were identified. A statistical analysis revealed significant associations between LGB, LGB-R genotypes as well as intragenic haplotypes LGB/LGB-R and milk protein content during the first complete lactation. Cows with AA LGB genotype, R3 LGB-R SSCP pattern and AA/R3 haplotypes had the highest protein content. These results support the hypothesis that sequence variation within the promoter of the LGB gene is probably one of the factors responsible for differences in milk protein content.
3

Genotyping of bovine beta-lactoglobulin (LGB) by PCR-SSCP technique

100%
Kaminski S., Zabolewicz T.
|
EN
A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.
4

Beta-lactoglobulin and kappa-casein polymorphism in relation to production traits and technological properties of milk in the herd of Polish Black-and-White cows

81%
Walawski K., Sowinski G., Czarnik U., Zabolewicz T.
Genetica Polonica
|
1994
|
vol. 35
|
issue 1-2
93-108
5

Effectiveness of a program aimed at the elimination of BLAD-carrier bulls from Polish Holstein-Friesian cattle

71%
Czarnik U., Grzybowski G., Kaminski S., Prusak B., Zabolewicz T.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 4
375-377
EN
The molecular basis of BLAD is the D128G mutation of the gene coding for the CD18 subunit of beta?2 integrin. This mutation is lethal, since homozygous (BL/BL) animals die before they reach sexual maturity. In the 1990s, BLAD was the most widespread genetic disease in HF cattle worldwide. The aim of the present study was to determine the frequency of BLAD carriers among 4645 young breeding bulls in Poland in 1995?2006. The frequency of carriers of the mutated allele showed a clear decreasing trend. The highest frequency (7.9%) was recorded while implementing the BLAD control program (1995?1997). Regular monitoring has enabled a great reduction of this threat to the tested population. Today only sporadic cases of BL/TL heterozygotes are reported (ca. 0.8% in 2004?2006).
6
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Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

71%
Kamiński S., Zabolewicz T., Barcewicz M., Brym P., Puckowska P.
Polish Journal of Veterinary Sciences
|
2014
|
issue 4
7

DGAT1 K232A quantitative trait nucleotide polymorphism in Polish Black-and-White cattle

71%
Pareek C. S., Czarnik U., Zabolewicz T., Pareek R. S., Walawski K.
Journal of Applied Genetics
|
2005
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vol. 46
|
issue 1
85-87
EN
The diacylglycerol o-acyltransferase 1 gene (DGAT1) was investigated in Polish Black-and-White cattle. The frequency of the K allele was 0.60, 0.68 and 0.48 for AI sires (n = 150), young bulls (n = 139) and cows (n = 213), respectively. The method of selective genotyping for identification of the quantitative trait nucleotide was verified through identification of DGAT1 effect on milk production traits. Daughters of six heterozygous bulls were selectively genotyped based on their milk traits. The genotypic frequencies differed between high and low yield groups representing milk and fat contents. The Kruskal-Wallis test revealed a highly significant effect of DGAT1 K232A in cows with extremely low fat content and a significant effect in cows with extremely high protein content of milk. No significant effect of AI sires' genotypes on their breeding value was found.
8
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Assessment of genomic inbreeding in Polish Konik horses

71%
Jaworski Z., Kamiński S., Hering D. M., Zabolewicz T., Ruść A.
Polish Journal of Veterinary Sciences
|
2017
|
issue 3
9

Silent point mutation polymorphism of the bovine CD18 encoding gene

71%
Czarnik U., Zabolewicz T., Galinski M., Pareek C. S., Walawski K.
Journal of Applied Genetics
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2004
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vol. 45
|
issue 1
73-76
EN
We report on a PCR-RFLP procedure for recognising of a silent point mutation of ITGB2 CD18 subunit gene in cattle. Polymorphism screening was performed in a Polish Black-and-White cattle population (n=210). The genotype and allele frequecies were established in the sires and cows. Further research is needed to explain the possible applications of the CD18 silent point mutation as a potential molecular marker for high milk productivity.
10

Deletion/insertion polymorphism of the prion protein gene (PRNP) in Polish Holstein-Friesian cattle

62%
Czarnik U., Zabolewicz T., Strychalski J., Grzybowski G., Bogusz M., Walawski K.
Journal of Applied Genetics
|
2007
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vol. 48
|
issue 1
69-71
EN
The aim of the present study was to identify the deletion/insertion polymorphism of the bovine prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3' untranslated region (14 bp). DNA was isolated from blood of 234 randomly tested Polish Holstein-Friesian cows and from semen of 47 sires used for artificial insemination (AI) in 2004. No statistically significant differences were found in the frequency of genotypes and alleles between cows and breeding bulls in the 3 analysed polymorphic sites within the PRNP gene. Only 3 haplotypes were identified in sires and 4 haplotypes in cows.
11

Deletion/insertion polymorphism of the prion protein gene (PRNP) in Polish Holstein-Friesian cattle

62%
Czarnik U., Zabolewicz T., Strychalski J., Grzybowski G., Bogusz M., Walawski K.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 3
69-71
EN
The aim of the present study was to identify the deletion/insertion polymorphism of the bovine prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3' untranslated region (14 bp). DNA was isolated from blood of 234 randomly tested Polish Holstein-Friesian cows and from semen of 47 sires used for artificial insemination (AI) in 2004. No statistically significant differences were found in the frequency of genotypes and alleles between cows and breeding bulls in the 3 analysed polymorphic sites within the PRNP gene. Only 3 haplotypes were identified in sires and 4 haplotypes in cows.
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