Search results

1

Fragments of LINE-1 retrotransposons flanked by inverted telomeric repeats are present in the bovine genome. Homology with human LINE-1 elements

100%

Płucienniczak G., Płucienniczak A.

Acta Biochimica Polonica

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1999

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vol. 46

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issue 4

873-878

EN

In the bovine genome we found two intrachromosomal DNA fragments flanked by inverted telomeric repeats (GenBank Accession Nos. AF136741 and AF136742). The internal parts of the fragments are homologous exclusively to the human sequences and to the consensus sequence of the L1MC4 subfamily of LINE-1 retrotransposons which are widespread among mammalian genomes. We found that distribution of homologous human sequences within our fragments is not random, reflecting a complicated pattern of insertion mechanisms of and maintenance of retrotransposons in mammalian genomes. One of the possible explanations of the origin of LINE-1 truncated elements flanked by inverted telomeric repeats in the bovine genome is that extrachromosomal DNA fragments may be modified by telomerase and subsequently, transferred into chromosomal DNA.

2

Hybridization of restricion fragments derived from calf satellite I DNA

81%

Sobieszczuk P., Furtak K., Skowroński J., Płucienniczak A.

Acta Biochimica Polonica

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1980

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vol. 27

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issue 3-4

303-308

3

Integrony

81%

Wolinowska R., Masny A., Płucienniczak A.

Kosmos

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2002

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vol. 51

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issue 3

353-364

EN

Summary Accumulation of antibiotic resistance among pathogenic bacteria has called attention to horizontal gene transfer that involves plasmids and transpozons. Integrons, usually placed on mobile genome elements, are very deeply engaged in the process of origin of multiple-drug-resistant strains. Integrons are genetic elements that contain determinants of the components of the site-specific recombination system that recognizes and captures mobile gene cassettes. More than 70 different antibiotic resistance genes covering most classes of antimicrobials presently in use have been detected in gene cassettes. Integrons are frequently found in clinical and environmental strains of gram-negative rods. The discovery of super-integrons, i.e. genetic structures gathering gene cassettes in a huge number, led to the conception of genome cassettes capture as an element of a broader phenomenon of bacterial genome modification in response to changing environmental conditions.

4

The factor VIII protein and its function

81%

Mazurkiewicz-Pisarek A., Płucienniczak G., Ciach T., Płucienniczak A.

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2016

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vol. 63

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issue 1

11-16

EN

Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. Human factor VIII is a single chain of about 300 kDa consisting of domains described as A1-A2-B-A3-C1-C2. The protein undergoes processing prior to secretion into blood resulting in a heavy chain of 200 kDa (A1-A2-B) and a light chain of 80 kDa (A3-C1-C2) linked by metal ions. The role of factor VIII is to increase the catalytic efficiency of factor IXa in the activation of factor X. Variants of these factors lead frequently also to severe bleeding disorders.

5

Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.

71%

Bicka L., Kuźmak J., Kozaczyńska B., Płucienniczak A., Skorupska A.

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2001

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vol. 48

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issue 1

227-232

EN

The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.

6

Adaptation of PCR technique for quantitative estimation of genetic material from different regions of chromosome 21 in cases of trisomy 21.

71%

Nowacka J., Helszer Z., Walter Z., Płucienniczak A., Kałużewski B.

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vol. 51

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issue 4

995-1001

EN

Pre- and postnatal diagnosis of chromosomal aberrations is generally based on conventional cytogenetic analysis. In this paper, we have devised a quantitative polymerase chain reaction (Q-PCR) method to determine gene dose effects and applied it in cases of regular trisomy 21 as a model. The method is based on quantitative assessment of PCR products after using primers amplifying DNA fragments located in the pericentromeric, heterochromatic, euchromatic and telomeric regions of chromosome 21. A gene dose effect on the amount of PCR product in cases of trisomy 21 was confirmed. Moreover, a correlation between the amount of the PCR product of the examined sequences and their location in the chromosome was observed. The obtained results suggest that the Q-PCR technique can be applied in the diagnosis of aneuploidies.

7

The effect of Arg209 to Lys mutation in mouse thymidylate synthase.

62%

Cieśla J., Gołos B., Wałajtys-Rode E., Jagielska E., Płucienniczak A., Rode W.

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2002

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vol. 49

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issue 3

651-658

EN

Mouse thymidylate synthase R209K (a mutation corresponding to R218K in Lactobacillus casei), overexpressed in thymidylate synthase-deficient Escherichia coli strain, was poorly soluble and with only feeble enzyme activity. The mutated protein, incubated with FdUMP and N5,10-methylenetetrahydrofolate, did not form a complex stable under conditions of SDS/polyacrylamide gel electrophoresis. The reaction catalyzed by the R209K enzyme (studied in a crude extract), compared to that catalyzed by purified wild-type recombinant mouse thymidylate synthase, showed the Km value for dUMP 571-fold higher and Vmax value over 50-fold (assuming that the mutated enzyme constituted 20% of total crude extract protein) lower. Thus the ratios kcat, R209K/kcat, 'wild' and (kcat, R209K/Km, R209KdUMP)/( kcat, 'wild'/Km, 'wild'dUMP) were 0.019 and 0.000032, respectively, documenting that mouse thymidylate synthase R209, similar to the corresponding L. casei R218, is essential for both dUMP binding and enzyme reaction.

8

In vitro phosphorylation of ex-preprotachykinin by protein kinase C

62%

Hrabec E., Hrabec Z., Lachowicz L., Greger J., Płucienniczak G., Płucienniczak A.

Acta Biochimica Polonica

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1994

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vol. 41

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issue 2

194-195

9

Molecular basis of cellulose biosynthesis disappearance in submerged culture of Acetobacter xylinum

62%

Krystynowicz A., Koziołkiewicz M., Wiktorowska-Jezierska A., Bielecki S., Klemenska E., Masny A., Płucienniczak A.

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2005

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vol. 52

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issue 3

691-698

EN

Acetobacter xylinum strains are known as very efficient producers of bacterial cellulose which, due to its unique properties, has great application potential. One of the most important problems faced during cellulose synthesis by these bacteria is generation of cellulose non-producing cells, which can appear under submerged culture conditions. The reasons of this remain unknow. These studies have been undertaken to compare at the molecular level wild-type, cellulose producing (Cel+) A. xylinum strains with Cel- forms of cellulose-negative phenotype. Comparison of protein profiles of both forms of A. xylinum by 2D electrophoresis allowed for the isolation of proteins which were produced exclusively by either Cel+ or Cel- cells. Sequences of peptides derived from these proteins were aligned with those of proteins deposited in databases. This analysis revealed that Cel- cells lacked two enzymes: phosphoglucomutase and glucose-1-phosphate uridylyltransferase, which generates UDP-glucose being the substrate for cellulose synthase. DNA was analyzed by ligation-mediated PCR carried out at low denaturation temperature (PCR-MP). Two DNA fragments of different thermal stability (218 and 217 bp) were obtained from the DNA of Cel+ and Cel- forms, respectively. The only difference between these Cel- and Cel+ DNA fragments is deletion of one T residue. Alignment of those two sequences with those deposited in the GenBank database revealed that similar fragments are present in the genomes of some bacterial cellulose producers and are located downstream from open reading frames (ORF) encoding phosphoglucomutase. The meaning of this observation is discussed.

10

The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E. coli, purification and ESR studies of its interaction with human blood platelets

52%

Pietrucha T., Stec W. J., Okruszek A., Uznański B., Koziołkiewicz M., Wilk A., Płucienniczak A., Świątkowska M., Cierniewski C. S.

Acta Biochimica Polonica

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1994

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vol. 41

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issue 1

25-34

Refine search results

Fragments of LINE-1 retrotransposons flanked by inverted telomeric repeats are present in the bovine genome. Homology with human LINE-1 elements

Hybridization of restricion fragments derived from calf satellite I DNA

Integrony

The factor VIII protein and its function

Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.

Adaptation of PCR technique for quantitative estimation of genetic material from different regions of chromosome 21 in cases of trisomy 21.

The effect of Arg209 to Lys mutation in mouse thymidylate synthase.

In vitro phosphorylation of ex-preprotachykinin by protein kinase C

Molecular basis of cellulose biosynthesis disappearance in submerged culture of Acetobacter xylinum

The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E. coli, purification and ESR studies of its interaction with human blood platelets