One of the optimization theory worked out by Ościk has been used for some physicochemical studies and optimization of chromatographic process. On the basis of the theoretical equations a retention in binary mobile phases can be predicted with good agreement for a lot of chromatographic systems. The influence of the different properties of chromatographic systems can be determined by use of the discussed optimization theory.The possibilities of the use of the theory for some physicochemical studies as determination of the hydrophobicity have been demonstrated too.
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