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Stimulation of anthocyanin production by two-phase growth system, in callus of Rudbeckia hirta

100%

Luczkiewicz M., Cisowski W.

Biotechnologia

1999

issue 4

199-210

Biotechnological research to achieve and then increase anthocyanin production in callus tissue of R. hirta L., involved testing a number of growth media, modified with growth regulators. The evaluation of the growth of propagated tissues, their water contents and in particular their ability to produce anthocyanins, led to the development of an original two-phase growth system. This new system has the advantages of both growth-stimulating media and pigment production media. As a result of the research, callus tissue was obtained on a two-phase growth medium, which was made of modified Schenk-Hildebrandt medium (growth phase) and Miller's medium (production phase). The callus synthesised a 12-component anthocyanin compound complex, which constituted 2,18% of dry mass. This is a considerable amount compared to 0,28% in the natural plant. Phytochemical analysis (TLC, HPLC, PC, UV, 1H-NMR) of the anthocyanin complex isolated from callus produced with the two-phase system proved that the dominating compounds in the pigment complex were: cyanidin-3-0-(6-0-malonyl-b -D-glucopyranoside) and cyanidin-3-0-(b-D-glucopyranoside).

Conditions for efficient induction and maintenance of callus cultures Vaccinium corymbosum var. Bluecrop and its accumulation of polyphenolic compounds

81%

Dembinska-Migas W., Luczkiewicz M., Migas P., Cisowski W.

Biotechnologia

1998

issue 2

174-185

Zimmerman & Bromme and Schenk & Hildebrandt media with unchanged content of plant growth regulators in the basic medium, were used to develop the optimum conditions for efficient production of callus tissue of Vaccinium corymbosum var. Bluecrop. It has been determined that, of the auxins used, it was 2,4-D which has the most beneficial effect on callus tissue growth. Zeatin was found to regulate the water content of the tissue obtained. Quercetin-3-O-glucoside, as well as quercetin-3-O-galactoside were identified and analysed quantitatively in the callus tissue from in vitro cultures. Moreover, the following phenolic acids were identified in the callus with the use of chromatography: ferulic acid, p-hydroxybenzoic acid, p-coumaric acid, as well as gallic acid and protocatechuic acid which were also analysed quantitatively in the researched tissue material. Phytochemical monitoring of the propagated tissues for different production media (HPLC analysis) did not show any relationship between the plant growth regulators used and the analysed secondary metabolites.

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2 Biotechnologia

1 1999

1 1998

2 Cisowski W.

2 Luczkiewicz M.

1 Dembinska-Migas W.

1 Migas P.

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Stimulation of anthocyanin production by two-phase growth system, in callus of Rudbeckia hirta

Conditions for efficient induction and maintenance of callus cultures Vaccinium corymbosum var. Bluecrop and its accumulation of polyphenolic compounds