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Circadian variations of histamine binding to lymphocytes and neutrophils and skin reactivity to histamine in atopic and healthy subjects

100%

Zak-Nejmark T., Nowak I. A., Kraus-Filarska M.

Archivum Immunologiae et Therapiae Experimentalis

2006

vol. 54

issue 4

283-287

Intruction: Numerous pathophysiological conditions change during 24-hour periods. Histamine, the main mediator in allergic reactions, exerts a multiplicity of pathophysiological actions through binding to specific receptors on effector cells. Nocturnal exacerbation of symptoms occurs in many atopic diseases in which histamine is an important mediator. Nocturnal wheezing is a very common symptom of asthma. The aim of this study was to determine whether the binding of (fluorescein-labeled) histamine to cells participating in allergic-inflammatory processes (lymphocytes, neutrophils) and skin reactivity to histamine undergo circadian changes and to compare these phenomena in atopic asthmatic and healthy subjects. Materials and Methods: Blood samples were collected at 8 am, 2 pm, 8 pm, 2 am, and 8 am the next day. Histamine skin-prick tests were performed at the same times. Results: It was found that skin reactivity to histamine (wheal, erythema) in healthy subjects underwent significant circadian changes with acrophase at 8 am (wheal) or 8 pm (erythema), the lowest values being at night (2 am, p=0.017), in contrast to atopics, in whom the highest reactivity was found at night (2 am, p=0.002). Significant differences in the binding of fluorescein-labeled histamine between day (8 am?2 pm) and night (2 am) were observed for lymphocytes (p=0.006) and neutrophils (p=0.018). Conclusions: In the asthmatic group these changes were not significant. Circadian changes in both the binding of histamine by effector cells and skin reactivity to histamine were different in healthy and asthmatic subjects, and this may play a role in the pathomechanism, course, and chronopharmacotherapy of atopic diseases.

Histamine receptor expression on peripheral blood lymphocytes is influenced by specific and nonspecific activation

76%

Zak-Nejmark T., Kraus-Filarska M., Malolepszy J., Jankowska R., Jutel M., Nowak I. A., Nadobna G.

vol. 48

issue 2

107-110

Histamine is a physiological mediator which exerts both effector and regulatory functions through its receptors on various cells. The aim of the study was to investigate changes in histamine receptor expression on peripheral blood lymphocytes affected by stimulation with both specific and nonspecific stimuli. Lymphocytes were obtained from both healthy and allergic subjects. Cells were incubated with various allergens (mixed grass pollen, Lolium perenne, Dermatophagoides pteronyssinus 1, bee venom, phospholipase A2) and nonspecific (fMLP, PMA/ionomycin, LPS) stimuli. The percentage of histamine-binding cells was determined with a fluorescence microscope after incubation with histamine-fluorescein. In control subjects histamine binding after stimulation with allergens was not significantly changed. In contrast, in allergic subjects stimulation with specific allergens resulted in significantly increased histamine binding. Nonspecific stimulation caused increased histamine binding to lymphocytes in both allergic subjects and healthy controls. We conclude that specific and nonspecific activation of lymphocytes is associated with increased expression of histamine receptors.

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2 Archivum Immunologiae et Therapiae Experimentalis

1 2006

1 2000

2 Kraus-Filarska M.

2 Nowak I. A.

2 Zak-Nejmark T.

1 Jankowska R.

1 Jutel M.

1 Malolepszy J.

1 Nadobna G.

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Circadian variations of histamine binding to lymphocytes and neutrophils and skin reactivity to histamine in atopic and healthy subjects

Histamine receptor expression on peripheral blood lymphocytes is influenced by specific and nonspecific activation