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OCT 4 immunohistochemistry in postpubertal cryptorchidism

100%

Krhen I., Kulis T., Coric M., Knezevic N., Marekovic Z., Kastelan Z.

Open Medicine

2011

vol. 6

issue 2

243-246

Patients with cryptorchidism are at an increased risk for germ cell testicular cancer. OCT 4 has been shown to be a sensitive and specific marker for some types of germ cell testicular cancer. We undertook this study to establish whether OCT 4 immunohistochemistry is a useful tool in the pathohistologic evaluation of postpubertal patients with cryptorchidism. Seventeen postpubertal patients underwent orchidectomy for cryptorchidism at our center since 1997. Immunohistochemical staining with OCT 4 was performed on these samples. Characteristic OCT 4 nuclear staining was positive in two patients. One patient was correctly diagnosed on previous pathohistological evaluation, while OCT4 immunohistochemical staining revealed previously unidentified intratubular germ cell neoplasia in the other patient. OCT 4 immunohistochemistry can be useful in diagnosing a testicular germ cell tumor in patients with cryptorchidism. If we consider a low number of postpubertal patients with cryptorchidism a benefit of immunohistochemical staining with OCT4, this could favor the use of OCT 4 staining in work-up of cryptorchidism.

Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells

68%

Cipak A., Borovic S., Jaganjac M., Bresgen N., Kirac I., Grbesa I., Mrakovcic L., Cindric M., Scukanec-Spoljar M., Gall-Troselj K., Coric M., Eckl P., Zarkovic N.

Acta Biochimica Polonica

2010

vol. 57

issue 2

185-191

Liver is a unique mammalian organ with a great capacity of regeneration related to its function. After surgical resection or injury, hepatic cells, especially hepatocytes, can proliferate rapidly to repair the damage and to regenerate the structure without affecting the function of the liver. Loss of catalase activity during regeneration indicates that oxidative stress is present in the liver not only in pathological conditions but also as a 'physiological' factor during regeneration. As we have shown in our previous work, liver stem cell-like cells treated with 4-hydroxynonenal (HNE), a cytotoxic and growth regulating lipid peroxidation product, recover in the presence of spleen cells. In the current study we characterized this novel cell line as liver-derived progenitor/oval-like cells, (LDP/OCs), i.e. functional liver stem-like cells. We showed that LDP/OC were OV6 positive, with abundant glycogen content in the cytoplasm and expressed α-fetoprotein, albumin, biliverdin reductase and γ-glutamyl transferase. Also, we compared their growth in vitro with the growth of cultured primary hepatocytes stressed with HNE and co-cultured with autologous spleen cells. The influence of spleen cells on HNE-treated primary hepatocytes and on LDP/OCs showed that spleen cells support in a similar manner the recovery of both types of liver cells indicating their important role in regeneration. Hence, LDP/OC cells may provide a valuable tool to study cell interactions and the role on HNE in liver regeneration.

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1 Acta Biochimica Polonica

1 Open Medicine

1 2011

1 2010

2 Coric M.

1 Borovic S.

1 Bresgen N.

1 Cindric M.

1 Cipak A.

1 Eckl P.

1 Gall-Troselj K.

1 Grbesa I.

1 Jaganjac M.

1 Kastelan Z.

1 Kirac I.

1 Knezevic N.

1 Krhen I.

1 Kulis T.

1 Marekovic Z.

1 Mrakovcic L.

1 Scukanec-Spoljar M.

1 Zarkovic N.

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OCT 4 immunohistochemistry in postpubertal cryptorchidism

Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells