Results found: 3

Number of results on page

Search results

Sort By:

Limit search:

Hormonal regulation of protein synthesis in the silk gland of Galleria mellonella

100%

Kłudkiewicz B., Grzelak K., Szołajska E., Michalik J.

Acta Biochimica Polonica

1991

vol. 38

issue 1

53-59

Influence of low temperature on the synthesis of some Galleria mellonella proteins

88%

Kłudkiewicz B., Godlewski J., Grzelak K., Cymborowski B., Lassota Z.

Acta Biochimica Polonica

1996

vol. 43

issue 4

639-644

Expression, purification and characterization of glycosylated influenza H5N1 hemagglutinin produced in Pichia pastoris

64%

Kopera E., Dwornyk A., Kosson P., Florys K., Sączyńska V., Dębski J., Cecuda-Adamczewska V., Szewczyk B., Zagórski-Ostoja W., Grzelak K.

2014

vol. 61

issue 3

597-602

The A/swan/Poland/305-135V08/2006 (H5N1-subtype) hemagglutinin (HA) gene was cloned and expressed in yeast Pichia pastoris (P. pastoris). The HA cDNA lacking the C-terminal transmembrane anchor-coding sequence was fused to an α-factor leader peptide and placed under control of the methanol-inducible P. pastoris alcohol oxidase 1 (AOX1) promoter. Two P. pastoris strains: SMD 1168 and KM 71 were used for protein expression. Recombinant HA protein was secreted into the culture medium reaching an approximately 15 mg/L (KM 71 strain). Fusion protein with a His6 tag was purified to homogeneity in one step affinity chromatography. SDS-PAGE and MS/MS analysis indicated that the protein is cleaved into HA1 and HA2 domains linked by a disulfide bond. Analysis of the N-linked glycans revealed that the overexpressed HA is fully glycosylated at the same sites as the native HA in the vaccine strain. Immunological activity of the hemagglutinin protein was tested in mice, where rHA elicited a high immune response.

Refine search results

3 Acta Biochimica Polonica

1 2014

1 1996

1 1991

3 Grzelak K.

2 Kłudkiewicz B.

1 Cecuda-Adamczewska V.

1 Cymborowski B.

1 Dwornyk A.

1 Dębski J.

1 Florys K.

1 Godlewski J.

1 Kopera E.

1 Kosson P.

1 Lassota Z.

1 Michalik J.

1 Szewczyk B.

1 Szołajska E.

1 Sączyńska V.

1 Zagórski-Ostoja W.

Search results

Hormonal regulation of protein synthesis in the silk gland of Galleria mellonella

Influence of low temperature on the synthesis of some Galleria mellonella proteins

Expression, purification and characterization of glycosylated influenza H5N1 hemagglutinin produced in Pichia pastoris