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Assessment of selected costimulatory, adhesion, and activatory molecules and cytokines of Th1/Th2 balance in acute lymphoblastic leukemia in children

100%

Luczynski W., Stasiak-Barmuta A., Krawczuk-Rybak M., Malinowska I.

Archivum Immunologiae et Therapiae Experimentalis

2005

vol. 53

issue 4

357-363

Recent years have seen a rise in the importance of cytokine production and co-stimulatory/activatory molecule expression in the immune response in leukemia. The aim of our study was to assess the function of T lymphocytes in children with acute lymphoblastic leukemia (ALL) during remission induction based on selected cytokine and co-stimulatory/activatory molecule expression. The study group consisted of 50 children with ALL (B cell precursor). Peripheral blood samples were taken before treatment (day 0), after the prednisone prophase (day 8), and during (day 15) and after (day 33) remission induction. The percentages of T cells with interferon (IFN)-g (Th1), interleukin (IL)-4 (Th2) and IL-2 receptor (IL-2R), CD28, CTLA-4, CD38, ICAM-1, and HLA-DR expression were assessed by tricolor flow cytometry. At the time of diagnosis we noted higher percentages of T cells with adhesion molecule ICAM-1, activation molecule CD38 expression, and an increased population of Th2 cells (IL-4) compared with the control group. During and after remission induction we observed a decreased population of CD38+ T cells, elevated percentages of helper T lymphocytes with IL-2R expression, and a rise in helper T lymphocytes producing IFN-g (Th1). During fever/infection, higher levels of activated T lymphocytes (CD4+HLA-DR+, CD8+HLA-DR+), a rise in Th1, and no change in Th2 populations were observed. The results suggest T cell activation and Th2 predominance at the time of diagnosis and during remission induction in ALL in children. These results confirm the involvement of cellular immunity in the leukemic process and can be used in immune therapy in leukemia.

CD80 and CD86 expression on LPS-stimulated monocytes and effect of CD80 and CD86 blockade on IL-4 and IFN-gamma production in nonatopic bronchial asthma

76%

Rutkowski R., Moniuszko T., Stasiak-Barmuta A., Kosztyla-Hojna B., Alifier M., Rutkowski K., Tatarczuk-Krawiel A.

CD80 and CD86 seem to play an important role in the allergen induced secretion of IL-5 and IL-13. Up till now, the expression of CD80 (B7.1) and CD86 (B7.2) on monocytes and kinetics of these molecules expression on lipopolysaccharide?stimulated monocytes in nonatopic asthma have not been defined. Using monoclonal antibodies we have compared the expression of CD80 (B7.1) and CD86 (B7.2) on monocytes of healthy persons and nonatopic asthmatic patients. We have also assessed the effect of CD80 and CD86 inactivation on interleukin (IL)-4 and interferon gamma (IFN-gamma production in nonatopic asthmatics and healthy subjects. We found that low expression of CD80 on studied monocytes (1.64+0.65 vs. 3.53+1.43%) and moderate expression of CD86 (41.25+134 vs. 49.46+11.49%) were characteristic for asthma. In nonatopic asthma patients inactivation of CD80 or CD86 blockade significantly reduced IFN-gamma production by T lymphocytes (p<0.02; p<0.03). In both studied groups anti-CD80 antibodies did not diminish T lymphocytes` production of IL-4. However anti-CD86 antibodies significantly (p<0.04) reduced the IL-4 concentration in culture supernatants. Our results confirm that both CD80 and CD86 molecules play on important role in the maintenance and amplification of inflammatory process. It suggests that in the inflammatory process that occurs in the nonatopic bronchial asthma Th1 as well as Th2 lymphocytes are equally important

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2 Archivum Immunologiae et Therapiae Experimentalis

1 2005

1 2003

2 Stasiak-Barmuta A.

1 Alifier M.

1 Kosztyla-Hojna B.

1 Krawczuk-Rybak M.

1 Luczynski W.

1 Malinowska I.

1 Moniuszko T.

1 Rutkowski K.

1 Rutkowski R.

1 Tatarczuk-Krawiel A.

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Assessment of selected costimulatory, adhesion, and activatory molecules and cytokines of Th1/Th2 balance in acute lymphoblastic leukemia in children

CD80 and CD86 expression on LPS-stimulated monocytes and effect of CD80 and CD86 blockade on IL-4 and IFN-gamma production in nonatopic bronchial asthma