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Investigations of As-S-Se thin films for use as inorganic photoresist for digital image-matrix holography

100%

Bulanovs A., Gerbreders V., Kirilovs G., Teteris J.

Open Physics

2011

vol. 9

issue 5

1327-1333

As-S-Se chalcogenide thin films are successfully employed in classical and dot-matrix holography as inorganic photoresists for obtaining a relief-phase hologram. However using these films for image-matrix hologram recording has not been studied due to some features of image-matrix technology. For the applied research of the optical properties of As-S-Se films an experimental device of digital image-matrix holographic recording based on 100 mW 405 nm semi-conductor laser and Spatial Light Modulator (SLM) has been created. The device has the following main parameters: 140 × 105 µm frame size; laser intensity during exposure 10 W/cm2. With the help of this device diffraction grating and security holograms were recorded on As-S-Se thin films. The work reported herein presents results of an experimental study of how diffraction efficiency (DE) of the received relief-phase holographic gratings depends on an exposure and period. Diffraction grating profiles and speed of etching corresponding to different exposure doses are shown. Hologram samples with DE = 65% have been received which allows for using chalcogenide film as alternative to organic photoresists in applied dot-matrix and image-matrix holography.

Novel luminescent dyes for confocal laser scanning microscopy used in Trematoda parasite diagnostics

52%

Kirilova E., Kecko S., Mežaraupe L., Gavarāne I., Pučkins A., Mickeviča I., Rubeniņa I., Osipovs S., Bulanovs A., Pupiņš M., Kirjušina M.

2018

vol. 65

issue 3

449-454

Benzanthrone derivates are now widely used in many industrial and scientific applications as dyes for polymers and textiles. In biochemical, biomedical and diagnostics investigations benzanthrone dyes are used as a lipophilic fluorescent probe since many benzanthrone derivates demonstrate bright fluorescence and they have ability to intercalate between membrane lipids. The aim of research presented here was to assess the luminescence ability of benzanthrone derivatives using microscopic visualization of biological objects. Accordingly, specimens of freshwater trematodes: Diplostomum spathaceum, Diplodiscus subclavatus and Prosotocus confusus, were stained by novel benzanthrone dyes using different fixatives. The samples were examined under a confocal laser scanning microscope. All of the dyes tested demonstrated good results for digestive and reproductive system visualization. Based on obtained results we conclude that benzanthrone dyes could be used for internal and external structure confocal laser scanning microscopic imaging of trematode specimens.

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1 Acta Biochimica Polonica

1 Open Physics

1 2018

1 2011

2 Bulanovs A.

1 Gavarāne I.

1 Gerbreders V.

1 Kecko S.

1 Kirilova E.

1 Kirilovs G.

1 Kirjušina M.

1 Mežaraupe L.

1 Mickeviča I.

1 Osipovs S.

1 Pupiņš M.

1 Pučkins A.

1 Rubeniņa I.

1 Teteris J.

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Investigations of As-S-Se thin films for use as inorganic photoresist for digital image-matrix holography

Novel luminescent dyes for confocal laser scanning microscopy used in Trematoda parasite diagnostics