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1

Demand for monitoring genetically modified rapeseed in Poland

100%
Wisniewska I., Linkiewicz A., Sowa S.
Biotechnologia
|
2006
|
issue 3
16-23
EN
Rapeseed (Brassica napus L.) is the most important oilseed plant in Poland. Genetically modified (GM) rapeseed is planted on 18% of total rapeseed area in the world. The most frequent genetic modifications in rapeseed gave transgenic lines that are herbicide tolerant or have altered fatty acid composition. Coexistence of conventional and GM rapeseed is difficult due to several facts related to biology of this species. Contamination of rapeseed conventional varieties with rapeseed GM seems to be unavoidable during flowering time, harvest, storage and transport. For this reason monitoring of GM rapeseed in Poland is very important. At the moment no validated method is available for quantification of GM in rapeseed. Therefore development and validation of qualitative methods for rapeseed transgenic lines in routine food and feed analysis are necessary.
2

In vitro regeneration and transformation of rye (Secale cereale L.)

100%
Sowa S., Sowa A., Zimny J.
Biotechnologia
|
2000
|
issue 4
88-92
EN
In vitro cultures are an integral part of plant transformation. Genetic manipulation can be performed only on a single cell level. Therefore in vitro culture and regeneration of plants from a single cell are very important for successful transformation In vitro culture of rye is more difficult to conduct than of others cereals. Difficulties with in vitro regeneration of rye seems to be the main factor limiting the development of rye transformation systems. Genetic transformation process includes three main steps, single cell transformation, selection of transgenic cells and regeneration of plants from single cells. Efficiency of each of these steps can influence the result of the transformation process. Therefore optimisation of those steps is very important.Transformation has been performed using the microprojecticle method and scutellum of rye embryos as a target. Two constructs have been used in cotransformation, pDB1 containing a marker bar gene (phosphinotricine acetylotransferase) for Basta herbicide resistance and a repoter uidA gen (beta-glucuronidase) and pAwact-Sec containing 196 bp fragment of a sec-1 gene in anti-sense orientation. Using pAWact-Sec construct we tried to block the expression of the endogenous gene sec-1 coding omega-secalin which is storage protein of rye grain. We were able to regenerate transgenic rye plants containing all introduced genes. The efficiency of sec-1 expression blocking was analysed by SDS-PAGE method. Among 50 analysed kernels of T1 generation we found 5 with lower omega-secalin level. Complete blocking of omega-secalin was not observed.
3

Molecular analysis in detection and identification of genetically modified organisms (GMO)

100%
Linkiewicz A., Wisniewska I., Sowa S.
Biotechnologia
|
2006
|
issue 3
44-52
EN
Several DNA and protein-based analytical methods for GMOs detection have been established so far. For the detection of GMOs at the DNA level, mostly PCR-based methods are used, whereas at protein level ELISA and lateral strips are predominant. The choice of target sequence motif is the most important factor controlling the specificity of the PCR method. This review summarizes most widely used GMO analysis technologies and point out new areas of analytical investigations.
4

Polish transgenic cereal crops

88%
Zimny J., Sowa S., Menke-Milczarek I., Czaplicki A., Sowa A.
Biotechnologia
|
2000
|
issue 4
80-87
EN
First transgenic cereal plants have been obtained in Poland seven years ago. Within the time other cereals like wheat, rye and barley have been also transformed. The prerequisite for that was a very efficient regeneration system by somatic embryogenesis. Generally the basic study on transgenic cereals are quite advanced but the question is how to include transgenic lines in to practical breeding process? Most of genes, promoters and transformation methods are patented and probably Polish breeders will never afford to buy the licences. Though there is a need to concentrate the future work in Polish institutes on identification and isolation of genes of interest. Than to transfer them to plants and register transgenic varieties. According to the Polish law it is allowed to carry out the field experiments, but it is not possible to register the plant variety.
5

Consequences of transformation of embryogenic Triticale cell

88%
Zimny J., Czaplicki A., Menke-Milczarek I., Oleszczuk S., Sowa S.
Biotechnologia
|
2000
|
issue 2
64-72
EN
Plants carrying foreign genes have been obtained for many crops including wheat, rice, maize, barley and Triticale. The most important aspect for practical breeding is the regeneration of whole plants from a specific cell possessing the desired agronomic properties. Particle bombardment provided the necessary breakthrough for the efficient transformation of cereals. Efficient regeneration is a prerequisite for all transformation techniques. The aim of the presented work was to study the progeny of transgenic plants of the allohexaploid cereal species Triticale. By combining an efficient regeneration system with the successful particle bombardment method we were able to obtain transgenic Triticale plants. Transgene expression was sometimes unstable and generally resulted in the decline of the expression, although some lines showing stable expression were also selected. In our laboratory several generations of androgenic doublehaploid transgenic lines have been regenerated and multiplicated. The integrated transgenes were detected in Triticale lines by in situ hybridisation method. The stability of trangenes has been studied on ten generations. A regeneration system from single cell to plant combined with microprojectile bombardment appeared to be the most efficient transformation method for Triticale. Numerous chimeric genes are now available for research. Some of these genes may appear useful in the future breeding of Triticale.
6
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Superconductivity in U-T Alloys (T = Mo, Pt, Nb, Zr) Stabilized in the Cubic γ-U Structure

76%
Sowa S., Kim-Ngan N., Krupska M., Paukov M., Tkach I., Havela L.
Acta Physica Polonica A
|
2016
|
vol. 130
|
issue 2
521-526
EN
Using ultrafast cooling (with the cooling rate up to 10⁶ K/s) helps to minimize the T-alloying concentration (T = Mo, Pt, Pd, Nb, Zr) necessary to retain the γ-U phase down to low temperatures. All investigated splat-cooled U-T alloys become superconducting with T_{c} in the range of 0.61-2.11 K. The bulk character of superconductivity can be concluded for some of the splats when comparing the specific-heat anomaly at T_{c} with the BCS theory prediction.
7
Content available remote

Study of Ti, V and Their Oxides-Based Thin Films in the Search for Hydrogen Storage Materials

64%
Tarnawski Z., Zakrzewska K., Kim-Ngan N.-T., Krupska M., Sowa S., Drogowska K., Havela L., Balogh A.
Acta Physica Polonica A
|
2015
|
vol. 128
|
issue 3
431-439
EN
Thin film series consisting of Ti, V, TiO₂ and V₂O₅ layer with different layer geometries, sequences and thicknesses have been prepared by the sputtering technique. The hydrogen depth profile of selected films upon hydrogen charging at 1 bar and/or hydrogenation at pressure up to 102 bar was determined by using secondary ion mass spectrometry and nuclear reaction analysis using a N-15 beam. The highest hydrogen storage with a concentration up to 50 at.% was found in the pure Ti and Ti-contained layer, while it amounts to around 30% in the metallic Ti-V-Ni layer. Hydrogen can diffuse through the TiO₂ layer without accumulation, but can be stored in the VO₂ layer in some cases. Hydrogen can remove the preferential Ti orientation in the films and induce a complete transition of V₂O₅ into VO₂ in the films.
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