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1

Cytolysins and homologous proteins produced by Gram-negative bacteria. II.Hemolysins of Aeromonas sp., Morganella sp., as well as homologous proteins of Gram-negative bacteria

100%
Rozalski A.
Postępy Higieny i Medycyny Doświadczalnej
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1996
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vol. 50
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issue 4
363-374
EN
This article presents the genetic determination, synthesis and activation of hemolysins produced by mentioned above bacteria, as well as the possible role of these toxins in the pathogenicity. All these cytolysins are related to RTX proteins presented in the previous paper. The short information about cytotoxic proteins from other Gram-negative bacteria (P. aeruginosa, K. pneumoniae, Vibrionaceae, G. vaginalis) and homologous proteins from E. chrysanthemi and R. leguminosarum biovar viciae are also included.
2

Cytolisins and homologous proteins produced by Gram-negative bacteria. I. Hemolysis and leukotoxins of Escherichia coli, Bordatella pertusis, Pasteurella haemolytica and Actinobacillus sp.

100%
Rozalski A.
|
EN
Cytolysins produced by Gram-negative bacteria belong to the pore forming bacterial proteins. A branch of these toxins represents RTX family of proteins. The RTX (repeats in toxin) family name has been proposed based on the common presence of tandem copies of a nine-amino acid repeat (L-X-G-G-X-G-(N/D)-D-X). The paper presents the genetics, synthesis, secretion and cytolytic activity of these toxins.
3

Structure of the O-polysaccharide of Proteus mirabilis O19 and reclassification of certain Proteus strains that were formerly classified in serogroup O19

45%
Perepelov A. V., Rozalski A., Bartodziejska B., Senchenkova S. N., Knirel Y. A.
Archivum Immunologiae et Therapiae Experimentalis
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2004
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vol. 52
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issue 3
188-196
EN
Bacteria of the genus Proteus are a common cause of urinary tract infections. The O-polysaccharide chain of their LPS (O-antigen) defines the serological specificity of these bacteria. Based on the immunospecificity of the O-antigens, two species, P. mirabilis and P. vulgaris, were classified into 49 O-serogroups, and more O-serogroups for strains of these species and P. penneri have been subsequently proposed. The lipopolysaccharide of P. mirabilis CCUG 19011 from serogroup O19 was degraded and under mildly acidic and mildly alkaline conditions. Polysaccharides thus obtained were studied by chemical methods, including O-deacetylation, sugar and methylation analyses, and 1H- and 13C-NMR spectroscopy. Antisera were obtained by immunization of New Zealand white rabbits with heat-killed bacteria. In serological studies, enzyme immunosorbent assay, passive hemolysis test, and inhibition of passive hemolysis were used.The following structure of the O-polysaccharide repeating unit was established. ->3)-beta-D-GlcpNAc-(1->3)alpha-D-GalpNAc4,6(R-Pyr)-(1->4)-alpha-D-GalpA-(1->3)-alpha-L-Rhap2Ac-(1-> where R-Pyr is (R)-1-carboxyethylidene (an acetal-linked pyruvic acid). This structure is significantly different from the O-polysaccharide structures of P. vulgaris, P. hauseri and P. penneri strains from the same Proteus serogroup O19. Conclusions: Based on immunochemical studies of the lipopolysaccharides, it is suggested 1) to keep P. vulgaris CCUG 4654 and P. penneri 31 in serogroup O19 as two subgroups, 2) to reclassify P. mirabilis CCUG 19011 into a new Proteus serogroup, O51, and 3) to classify serologically related strains, including P. vulgaris ATCC 49990, P. hauseri 1732-80 and 1086-80, P. penneri 15, and some other P. penneri strains, in yet another Proteus serogroup, O52. This structure is significantly different from the O-polysaccharide structures of P. vulgaris, P. hauseri and P. penneri strains from the same Proteus serogroup O19. Based on immunochemical studies of the lipopolysaccharides, it is suggested 1) to keep P. vulgaris CCUG 4654 and P. penneri 31 in serogroup O19 as two subgroups, 2) to reclassify P. mirabilis CCUG 19011 into a new Proteus serogroup, O51, and 3) to classify serologically related strains, including P. vulgaris ATCC 49990, P. hauseri 1732-80 and 1086-80, P. penneri 15, and some other P. penneri strains, in yet another Proteus serogroup, O52.
4

Characterization and serological classification of O-specific polysaccharide of Proteus mirabilis TG 276-90 from Proteus serogroup O34

45%
Kolodziejska K., Siwinska M., Zych K., Rozalski A., Sidorczyk Z.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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vol. 54
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issue 3
223-226
EN
Introduction: Gram-negative bacteria of the genus Proteus from the family Enterobacteriaceae are currently divided into the five species P. mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens and three unnamed Proteus genomospecies 4, 5, and 6. They are important facultative human and animal pathogens which, under favorable conditions, cause mainly intestinal and urinary tract infections, sometimes leading to serious complications such as acute or chronic pyelonephritis and the formation of bladder and kidney stones. In this study we report on the serological properties of the lipopolysaccharide (LPS) of Proteus mirabilis TG 276-90, whose O-polysaccharide chemical structure was described earlier. Materials and Methods: LPS and alkali-treated LPS of a few serologically related Proteus strains and O-antisera against P. mirabilis TG 276-90 and CCUG 4669 (O34) were used. Serological characterization of P. mirabilis TG 276-90 O-specific polysaccharide was done using enzyme immunosorbent assay, passive immunohemolysis test (PIH), inhibition of these tests, SDS/PAGE and Western blot techniques, absorption of rabbit polyclonal O-antisera, and repeated PIH test. Results: Structural and serological investigations showed that the O-polysaccharides of P. mirabilis TG 276-90 and P. vulgaris O34 are identical and that their LPSs differ only in epitopes in the core part. Therefore these two strains could be classified into the same Proteus O34 serogroup. Conclusions: The serological data showed that the beta-D-GalpNAc-(14)-alpha-D-GalpNAc disaccharide is an important epitope of the P. mirabilis TG 276-90 and P. vulgaris O34 LPSs, shared by the P. mirabilis O16 and P. vulgaris TG 251 LPSs. It is responsible for cross-reactions with P. mirabilis TG 276-90 and P. vulgaris O34 O-antisera.
5

Serological characterization of the O-specific polysaccharide of Providencia alcalifaciens O23

45%
Torzewska A., Kocharova A. N., Maszewska A., Knirel Y. A., Rozalski A.
Archivum Immunologiae et Therapiae Experimentalis
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2004
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vol. 52
|
issue 1
43-49
EN
The genus Providencia belongs to the Enterobacteriaceae family and currently consists of five species: P.alcalifaciens, P.heimbachae, P.rettgerii, P.rustigianii and P.stuartii .The serological classification scheme of P.alcalifaciens, P.rustigianii and P.stuartii includes 63 O-serogroups and 30 H-serogroups. The O-antigenic specificity is defined by the structure of the O-antigen(O-specific polysaccharide ?OPS), a part of the lipopolysaccharide (LPS,endotoxin),one of the major components of the outer membrane of Gram-negative bacteria and an important virulence factor of these bacteria. Among the bacteria of the Enterobacteriaceae family, the genus Providencia is one of the least studied in respect to its LPS structure and antigenic specificity. Studies of the chemical structures and the serological specificity of the O-antigens aim at the elucidation of the molecular basis of the serological classification of Providenciasp. LPS and alkali-treated LPS of P.alcalifaciens O23 and serologically related P.rustigianii O14, P.mirabilis O13 and P.myxofaciens as well as O-antiserum against P.alcalifaciens O23 were used. Serological characterization of P.alcalifaciens O23 O-specific polysaccharide was done by use enzyme immunosorbent assay (EIA), passive hemolysis test (PHT)as well as by inhibition and sodium deoxycholate polyacrylamide gel electrophoresis (DOC-PAGE)of LPS and Western blot. The OPS of P.alcalifaciens,O23,contains an N-(D-glucuronoyl)-N-[(R)-1-carboxyethyl ]-L- lysine residue (GlcAAlaLys).The LPS of P.alcalifaciens,O23,and other LPSs containing AlaLys from Providencia and Proteus strains were tested with rabbit anti-P.alcalifiaciens O23 serum. The serological data showed that a GlcAAlaLys-associated epitope plays a role as an antigenic determinant in the P.alcalifaciens O23 OPS and revealed the particular importance of glucuronic acid and the carboxyethyl group for the binding of O23-specific antibodies.
6

Immunochemical studies on the O-antigens of Proteus mirabilis O23 and Proteus vulgaris O23

39%
Rozalski A., Perepelov A. V., Bartodziejska B., Senchenkova S. N., Babicka D., Kaca W., Knirel Y. A.
Archivum Immunologiae et Therapiae Experimentalis
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2003
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vol. 51
|
issue 1
69-73
EN
Analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy demonstrated that the O-specific polysaccharides of P. mirabilis PrK 42/57 and P. vulgaris PrK 43/57 are structurally similar to that of P. vulgaris PrK 44/57 and different from the polysaccharide of P. mirabilis PrK 41/57 studied earlier. The lipopolysaccharides of these strains were tested using enzyme immunosorbent assay, passive hemolysis and Western blot with O-antisera against P. mirabilis 42/57 and P. vulgaris 43/57 and 44/57, as well as with cross-absorbed O-antisera. The chemical and serological data revealed the basis for combining the four strains into Proteus serogroup O23 and division of this serogroup to three subgroups, one for P. vulgaris 43/57 and 44/57 and two others for P. mirabilis 41/57 and 42/57.
7

Structures and serology of the O-antigens of Proteus strains classified into serogroup O17 and former serogroup O35

33%
Torzewska A., Grabowski S., Kondakova A. N., Toukach F. V., Senchenkova S. N., Shashkov A. S., Arbatsky N. P., Knirel Y. A., Rozalski A., Kaca W.
Archivum Immunologiae et Therapiae Experimentalis
|
2006
|
vol. 54
|
issue 4
277-282
EN
Introduction: Bacteria of the genus Proteus are facultative pathogens which commonly cause urinary tract infections. Based on the serological specificity of the O-chain polysaccharide of the lipopolysaccharide (O-polysaccharide, O-antigen), strains of P. mirabilis and P. vulgaris have been classified into 60 serogroups. Studies on the chemical structure and serological specificity of the O-antigens aim at the elucidation of the molecular basis and improvement of the serological classification of these bacteria. Materials and Methods: The O-polysaccharide was prepared by acetic acid degradation of the lipopolysaccharide isolated from dried bacterial mass of each strain by hot phenol/water extraction. 1H- and 13C-NMR spectroscopy was used for structural studies. Serological studies were performed with rabbit O-antisera using enzyme immunosorbent assay, passive hemolysis test, and the inhibition of reactions in these assays as well DOC-PAGE and Western blot. Results: Four Proteus strains belonging to serogroups O17 and O35 were found to possess similar O-polysaccharide structures, in particular having the same carbohydrate backbone built up of tetrasaccharide repeating units. However, they differ in the presence or absence of additional substituents, such as phosphoethanolamine in P. mirabilis O17 and glucose in P. penneri O17, as well as in the pattern and degree of O-acetylation of various monosaccharide residues. Serological studies also showed close relationships between the O-antigens studied. Conclusions: Based on these data it is proposed to reclassify strain P. mirabilis PrK 61/57, formerly representing the O35 serogroup, into the serogroup O17 in the Kauffman-Perch classification system of Proteus.
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