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Contributions of the Hfq protein to translation regulation by small noncoding RNAs binding to the mRNA coding sequence

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Wroblewska Z., Olejniczak M.

2016

vol. 63

issue 4

701-707

The bacterial Sm-like protein Hfq affects the regulation of translation by small noncoding RNAs (sRNAs). In this way, Hfq participates in the cell adaptation to environmental stress, regulation of cellular metabolism, and bacterial virulence. The majority of known sRNAs bind complementary sequences in the 5'-untranslated mRNA regions. However, recent studies have shown that sRNAs can also target the mRNA coding sequence, even far downstream of the AUG start codon. In this review, we discuss how Hfq contributes to the translation regulation by those sRNAs which bind to the mRNA coding sequence.

The structure of fadL mRNA and its interactions with RybB sRNA

81%

Groszewska A., Wroblewska Z., Olejniczak M.

2016

vol. 63

issue 4

835-840

Small bacterial RNAs (sRNAs) regulate translation by pairing with complementary sequences in their target mRNAs, in a process which is often dependent on the Hfq protein. Here, the secondary structure of a 95-nt long fragment of Salmonella fadL mRNA containing RybB sRNA binding site in the coding region was analyzed. The data indicated local rearrangements in this mRNA structure after the annealing of RybB. The filter retention data had shown that Hfq bound both RybB and the fadL mRNA fragment with tight affinities. Moreover, Hfq increased the rate of RybB annealing to fadL mRNA. These data indicate that Hfq directly participates in RybB interactions with the fadL mRNA.

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2 Acta Biochimica Polonica

2 2016

2 Olejniczak M.

2 Wroblewska Z.

1 Groszewska A.

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Contributions of the Hfq protein to translation regulation by small noncoding RNAs binding to the mRNA coding sequence

The structure of fadL mRNA and its interactions with RybB sRNA