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  1. 2 Biotechnologia

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  1. 1 2010

  2. 1 2003

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  1. 2 Ptak A.

  2. 1 Cierniak O.

  3. 1 Gadek J.

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1

Micropropagation of Leucojum aestivum L. in bioraector RITA?

100%
Ptak A., Gadek J.
Biotechnologia
|
2010
|
issue 2
165-171
EN
Embryogenic callus of Leucojum aestivum was transferred to solid and liquid media in bioreactor RITA? vessels. Callus growth on the liquid medium enriched with 2 and 5 M Picloram and 0.5 M BA was characterized by a higher multiplication index. The greatest number of somatic embryos was observed on the callus multiplied in bioreactor using 5-minute flushing with the medium. Globular embryos developed into torpedo-stage embryos and plantlets under the influence of NAA (0.5 M) and zeatin (5 M). Liquid medium promoted the development of L. aestivum somatic embryos and plantlets.
2

Regeneration of summer snowflake (Leucojum aestivum L.) in in vitro cultures

100%
Ptak A., Cierniak O.
Biotechnologia
|
2003
|
issue 4
239-245
EN
Slices of scales and leaves isolated from Leucojum aestivum L. bulbs were cultured in the presence of auxins (NAA, 2,4-D, Picloram) and cytokinin, BA at different proportions and concentrations. The medium free of growth regulators was also used. Regeneration occurred as a result of somatic embryogenesis and organogenesis. Explants cultivated on the media containing 2,4-D and Picloram showed a capacity of somatic embryogenesis, viz. formation of embryogenic callus and somatic embryos. On the other hand, direct organogenesis (induction of bulblets and roots) occurred in explants grown on the medium devoid of growth regulators and enriched in NAA.
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