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Purification of geranylgeranyl diphosphate synthase from bovine brain

100%

Sagami H., Morita Y., Korenaga T., Ogura K.

Acta Biochimica Polonica

1994

vol. 41

issue 3

293-302

Dolichols of the fern Matteucia struthiopteris.

85%

Wojtas M., Bieńkowski T., Zelman-Femiak M., Tateyama S., Sagami H., Chojnacki T., Danikiewicz W., Swiezewska E.

2005

vol. 52

issue 1

255-259

Dolichols isolated from leaves of the fern Matteucia struthiopteris were present as a mixture of prenologues composed of 14 up to 20 isoprene units with Dol-16 dominating. They comprised approximately 0.004% of the fresh weight of fresh plant tissue and were accompanied by traces of polyprenols (Pren-14 up to Pren-17, Pren-16 dominating). Their structure was confirmed by electropray ionization mass spectrometry (ESI-MS). This is the first time that dolichols have been reported as dominating polyisoprenoid alcohols in plant photosynthetic tissue.

Formation of lipid droplets induced by 2,3-dihydrogeranylgeranoic acid distinct from geranylgeranoic acid

85%

Kodaira Y., Kusumoto T., Takahashi T., Matsumura Y., Miyagi Y., Okamoto K., Shidoji Y., Sagami H.

2007

vol. 54

issue 4

777-782

Geranylgeranoic acid (GGA) and 2,3-dihydrogeranylgeranoic acid (2,3-diGGA) are geranylgeraniol-derived metabolites (Kodaira et al. (2002) J Biochem 132: 327-334). In the present study, we examined the effects of these acids on HL-60 cells. The cells were differentiated into neutrophils by GGA stimulation like retinoic acid stimulation. In the case of cells stimulated with 2,3-diGGA, neutrophils were not detected, but the formation of lipid droplets was induced. On the other hand, when the cells were cultured in the presence of 0.1% FBS instead of 10% FBS, apoptotic cells were induced not only by GGA stimulation but also with 2,3-diGGA. In the latter case, when the cells were cultured in the co-presence of a caspase-3 inhibitor (Ac-DMQD-CHO), the lipid droplets formation was observed in the cells. These results suggest that GGA and 2,3-diGGA are extremely different from each other with respect to their effects on HL-60 cells.

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3 Acta Biochimica Polonica

1 2007

1 2005

1 1994

3 Sagami H.

1 Bieńkowski T.

1 Chojnacki T.

1 Danikiewicz W.

1 Kodaira Y.

1 Korenaga T.

1 Kusumoto T.

1 Matsumura Y.

1 Miyagi Y.

1 Morita Y.

1 Ogura K.

1 Okamoto K.

1 Shidoji Y.

1 Swiezewska E.

1 Takahashi T.

1 Tateyama S.

1 Wojtas M.

1 Zelman-Femiak M.

Search results

Purification of geranylgeranyl diphosphate synthase from bovine brain

Dolichols of the fern Matteucia struthiopteris.

Formation of lipid droplets induced by 2,3-dihydrogeranylgeranoic acid distinct from geranylgeranoic acid