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1

Uses of monoclonal antibodies in medicine

100%
Zielinski M., Plucienniczak A.
Biotechnologia
|
2009
|
issue 2
113-122
EN
This review article describes uses of monoclonal antibodies (mAb) in medicine as therapeutics. It contains information about the method of monoclonal antibodies production, types of their modifications and nomenclature. We show examples of mAb application in therapies.
2

Methods for isolation, purification and renaturation of recombinant proteins obtained in Escherichia coli bacteria

100%
Szczepanek A., Plucienniczak A.
Biotechnologia
|
1994
|
issue 4
102-125
EN
Escherichia coli is the most useful bacterial species applied to genetic engineering in recombinant proteins production process.The supply of many polipeptides with potential clinical or industrial use is often limited by their low natural availability. Overexpressed polipeptides may either be located in the cytoplasm and periplasm of E.coli or secreted through the cell membrne into the growth medium.Foreign proteins can be expressed in E.coli cells directly or as fusion proteins with prokaryotic sequences. Frequently, the overexpressed proteins acumulate in the bacterial cytoplasm or periplasm in the form of insoluble inclusion bodies.This review considers isolation, purification, solubiliztion and renaturation of recombinant proteins from E.coli, which is still a serious methodological and technical renaturtion.
3

THe methods for diagnosis of HIV infection

100%
Szczepanek A., Plucienniczak A.
Postępy Higieny i Medycyny Doświadczalnej
|
1994
|
vol. 48
|
issue 4
325-344
EN
The article contains a survay of methods for diagnosis of HIV infection based on detection of various viral markers:antibodies against HIV proteins, HIV-neutralizing antibodies, HIV p24 antigen, fragments of HIV genome or whole virus particles.
4

Clostridium botulinum toxin - a wonderful poison

100%
Mazurkiewicz-Pisarek A., Plucienniczak A.
Biotechnologia
|
2009
|
issue 2
123-133
EN
Botulinum toxin is a neurotoxin protein produced by the bacterium Clostridium botulinum. It is one of the most poisonous, naturally occurring substances in the world and it is the most toxic protein. Although it is highly toxic, it is used in medicine. Clinical applications of botulinum toxin, which include the treatment of overactive skeletal and smooth muscles, hypersecretory and painful disorders as well as cosmetics applications, have increased exponentially since it was first used clinically to treat strabismus more than two decades ag go. This paper describes the structure, mechanism of action and application of botulinum toxin.
5

Agrobacterium-mediated transformation of yellow lupin to generate callus tissue producing HBV surface antigen in a long-term culture

81%
Pniewski T., Kapusta J., Plucienniczak A.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 4
309-318
EN
The idea of an oral vaccine administered as a portion of plant tissue requires a high level of antigen production. An improved protocol for the induction of transgenic yellow lupin calli or tumours, reaching 44% of transformation rate, is presented here. It has been developed by using the nptII marker gene and the uidA reporter gene as well as various Agrobacterium strains and plant explants. This method of seedling and hypocotyl transformation was applied to raise calli or tumours producing a small surface antigen of Hepatitis B Virus (S-HBsAg). Lupin tissue lines were long-term cultured on selection media maintaining the growth rate and high expression level of the native form of S-HBs, up to 6 mug per g of fresh tissue.
6

Chromosomal localization of a novel repetitive sequence in the Chenopodium quinoa genome

81%
Kolano B., Plucienniczak A., Kwasniewski M., Maluszynska J.
Journal of Applied Genetics
|
2008
|
vol. 49
|
issue 4
313-320
EN
In this study, a novel repetitive sequence pTaq10 was isolated from the Taq I digest of the genomic DNA of the pseudocereal Chenopodium quinoa. Sequence analysis indicated that this 286-bp monomer is not homologous to any known retroelement sequence. FISH and Southern blot analysis showed that this sequence is characterized by an interspersed genomic organization. After FISH, hybridization signals were observed as small dots spread throughout all of the chromosomes. pTaq hybridization signals were excluded from 45S rRNA gene loci, but they partly overlapped with 5S rDNA loci. pTaq10 is not a species-specific sequence, as it was also detected in C. berlandieri.
7

Expression of exogenous genes in blastodermal cells of chicken in vitro

61%
Bednarczyk M., Plucienniczak G., Plucienniczak A., Lakota P., Sochanik A., Dluzniewska A., Grajewski B.
Folia Biologica
|
2003
|
vol. 51
|
issue 3-4
189-194
EN
Chicken blastodermal cells (BCs) from stage X embryos produce both somatic and germline chimeras when injected into the subgerminal cavity of recipient embryos. Transfection of the donor cells in vitro could lead to the production of chimeras capable of transmitting the transgene to their offspring. The aim of this study was to transfer and express foreign genes under control of the ovalbumin promoter in the BCs. The results showed that luciferase activity in the BCs reached a plateau value with a 2.0:1.0 or 5.0:1.0 liposome ? DNA ratio and using 1mug of DNA. Under this same condition, no difference was found in relative activity between the pGL-control and pOVALUC plasmid. The expression of other exogenous genes (green fluorescent protein and interferon alpha2a) driven by the chicken ovalbumin promoter in cultured chicken blastodermal cells in vitro is possible by this assay. Hatchability of recipient embryos after injection of 1, 500 or 800 transfected BCs was compared. The advantage of using a smaller number (800) of injected transfected BCs was that early embryonic mortality was reduced and resulted in higher (P<0.01) hatchability (24.5%) than in the case of 1, 500 BCs injected.
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