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1

The effect of concentration of mineral salts, kind of solidifying agent, cytokinin and TIBA on adventitious regeneration of apple rootstock P.59

100%
Wiejacha K., Orlikowska T.
Biotechnologia
|
2004
|
issue 2
191-198
EN
The aim of this investigation was to establish the conditions for effective regeneration of adventitious shoots on leaf explants of apple dwarf rootstock P.59. A three stage procedure was used ? the induction on medium containing thidiazuron (TDZ) and -naphtalene acetic acid (NAA) for two weeks, then the regeneration on medium supplemented with 6-benzylaminopurine (BAP) and NAA and growth of adventitious shoots on medium with low concentration of BAP and indole-3-butyric acid (IBA). The youngest leaves from proliferating shoot cultures were taken as explants for adventitious regeneration. The effect of MS salt concentration and type of solidifying agent in the induction and regeneration media, and a type of cytokinin in the induction medium was studied. It was proved that the regeneration potential was higher when 1/2 MS salts, Gelrite and TDZ was used.
2

Phytophthora ? pathogens of trees and shrubs. Detection and identification

81%
Wiejacha K., Szkuta G., Orlikowski L. B., Orlikowska T.
Biotechnologia
|
2004
|
issue 3
55-68
EN
The paper summarizes information on the raising up menace of ornamental and forest nurseries by Phytophthora spp. caused mostly by P. cinnamomi, P. citricola, P. citrophthora, P. cryptogea, P. ramorum. Some Phytophthora species are especially detrimental, but their identification is not possible on the basis of symptoms. To detect and identify pathogens in the plants (with- and without symptoms), soil and water, reliable tests are required. The survey on methods and techniques reported in the literature which can be used to establish such assays is given. The most needed are the tests based on DNA markers, especially when organized as micro-chips due to the large number of samples which can be screened in a short time.
3

Genomic polymorphism of isolates of Phytophthora ramorum from Polish ornamental nurseries compared with other European and North American isolates

71%
Wiejacha K., Trzewik A., Orlikowski L. B., Szkuta G., Orlikowska T.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 4
413-419
EN
We undertook an analysis of the genomic relationships between 15 isolates of Phytophthora ramorum Werres, de Cock et Man in't Veld, obtained from symptomatic plants growing in Polish ornamental nurseries, and 2 representatives of the European population and 3 of the North American population. Dendrograms were generated by UPGMA based on 786 amplification products obtained in ISSR-PCR reactions. The representatives of the European population and 13 of the 'Polish' isolates formed a common cluster. The other 2 ?Polish' isolates, which were found in 1998, and the 3 American representatives formed 2 separate clusters. There was no observed link between genomic distance on the basis of polymorphism and the origin of the isolates from plant species.
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