Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 2

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1
Publication available in full text mode
Content available

The usage of antibiotics for prevention of contamination in 3T3-L1 preadipocyte culture

100%
Ariyanto E. F., Wikayani T. P., Qomarilla N.
|
|
vol. 26
218-223
EN
Background: 3T3-L1 preadipocyte is the most commonly used cell line in in vivo studies of obesity. One of the main concerns in 3T3-L1 preadipocyte culture is microorganism contaminations. The objective of this study was to determine the appropriate antibiotics to prevent contaminations in 3T3-L1 cultures. Method: This study used descriptive analysis. Frozen 3T3-L1 preadipocytes were thawed and cultured in DMEM-10% FBS-1% penicillin-streptomycin, DMEM-10% FBS-1% penicillin-streptomycin-fungiezone, or DMEM-10% FBS-0.2% ciprofloxacin 200 mg/100 ml. After 24-hour incubation, the cells were observed under the microscope for any change in the medium colour, presence of abnormal structures, and abnormality in cell morphology. Results: The usage of 1% penicillin-streptomycin, 1% penicillin-streptomycin-fungiezone, or 0.2% ciprofloxacin 200 mg/100 ml maintained the clean medium and conserved normal fibroblast-like morphology of the cells. Conclusion: This study suggested that 1% penicillin-streptomycin, 1% penicillin-streptomycin-fungiezone, or 0.2% ciprofloxacin 200 mg/100 ml can be utilized in 3T3-L1 preadipocyte cultures to prevent contaminations.
2
Publication available in full text mode
Content available

Seeding cell number required for optimal lipid accumulation during adipocyte differentiation using 3T3-L1 cell line

76%
Ariyanto E. F., Odaiyappan N. A. L. S., Lidyana L., Wikayani T. P., Qomarilla N., Wira D. W., Triatin R. D.
World News of Natural Sciences
|
2019
|
vol. 25
220-226
EN
Obesity is one of the major causes of metabolic diseases such as diabetes and heart attack, and, hence, can lead to low quality of life. Elaborating adipocyte differentiation is very crucial for formulating the treatment and prevention of obesity. The objective of this study is to investigate the seeding cell number required to obtain optimum lipid accumulation during adipocyte differentiation using the 3T3-L1 cell line. Two sets of 5.48×104 (for Day 0 and Day 8 of differentiation), of 10.96×104 (for Day 8) and of 21.92×104 (for Day 8) of 3T3-L1 cells were seeded in each wells of a 12-well plate. Isobuthylmethylxanthine (IBMX), Dexamethasone, and Insulin-containing differentiation cocktails was added into the medium at Day 0 for 48 hours. The medium was changed every two days. Day 0 and Day 8 samples were then stained using Oil Red O and were examined under the microscope to observe the lipid droplets (red-coloured). The lipid droplets were quantified by measuring the absorbance at wavelength of 550 nm. In the study, seeding the number of 10.96×104 cells produced very significantly higher lipid accumulation, as compared with seeding the number of 5.48×104 cells. However, doubling the seeding number into 21.92×104 cells did not increase the lipid droplets significantly. This study found that the optimum seeding number to obtain the maximum lipid droplets during 3T3-L1 adipocyte differentiation was 10.96×104 cells.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.