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Study aim: To assess the relations between anthropometric and cardio-respiratory indices, and aerobic capacity of students, differing in the level of physical activity, under resting and exercise conditions.Material and methods: A group of 87 male and 75 female students volunteered to participate in the study. Their physical activity was evaluated by Seven-Day Physical Activity Recall questionnaire. Anthropometric (body height and mass, body fat content, BMI and WHR) and physiological indices (heart rate, blood pressure, O2max, CO2 and minute ventilation) were recorded. Body fat content was determined using a BIA device; respiratory data were recorded in a cycle ergometer test. The subjects were classified by their O2max values into ‘high’ or ‘low’ categories, the cut-off values of lower and upper quartiles serving as criteria.Results: Male and female students expended 10.2 ± 4.6 and 8.4 ± 5.3 kcal/kg/day, respectively, the O2max amounting to 48.4 ± 6.4 and 41.1 ± 4.7 ml/kg/min, respectively. Subjects having high O2max had significantly higher energy expenditure on physical activities, fat-free mass, body water content and maximal ventilation, and lower body mass, BMI, body fat content, resting heart rate and diastolic pressure.Conclusions: When investigating into the relationships between physical activity and physiological features, the latter ought to be related to O2max rather than to energy expenditure which may depend on other than physiological variables.
EN
Study aim: To evaluate plasma homocysteine (Hcy), insulin and glucose levels in blood and the insulin resistance index (FIRI) in young, healthy non-obese men and women.Material and methods: A total of 152 young, healthy, non-obese (BMI<30) men (n = 81) and women (n = 71) participated in the study. The following substances were assayed in blood using commercial kits: total plasma homocysteine by fluorescence polarisation immunoassay, plasma glucose - by the oxidase method, and insulin by radioimmunoassay using monoclonal antibodies. From the latter two, the index of insulin resistance (FIRI) was computed.Results: Mean plasma homocysteine concentration in men was significantly (p<0.001) higher than in women (10.3 ± 3.0 and 8.4 ± 2.4 μmol/l, respectively) and that of FIRI was significantly (p<0.001) lower than in women (1.310 ± 0.483 and 1.437 ± 0.420, respectively). Neither in men nor in women were plasma homocysteine concentrations correlated with FIRI.Conclusions: Although no association between circulating homocysteine and FIRI was found in young, non-obese men and women, the existence of such association in Type 2 diabetes cannot be ruled out.
EN
We hypothesized that the ACE ID / ACTN3 R577X genotype combination was associated with sprint and endurance performance. Therefore, the purpose of the present study was to determine the interaction between both ACE ID and ACTN3 R577X polymorphisms and sprint and endurance performance in swimmers. Genomic DNA was extracted from oral epithelial cells using GenElute Mammalian Genomic DNA Miniprep Kit (Sigma, Germany). All samples were genotyped using a real-time poly- merase chain reaction. The ACE I/D and the ACTN3 R577X genotype frequencies met Hardy-Weinberg expectations in both swimmers and controls. When the two swimmer groups, long distance swimmers (LDS) and short distance swimmers (SDS), were compared with control subjects in a single test, a significant association was found only for the ACE polymorphism, but not for ACTN3. Additionally, four ACE/ACTN3 combined genotypes (ID/RX, ID/XX, II/RX and II/XX) were statistically significant for the LDS versus Control comparison, but none for the SDS versus Control comparison. The ACE I/D and the ACTN3 R577X polymorphisms did not show any association with sprint swimming, taken individually or in combination. In spite of numerous previous reports of associations with athletic status or sprint performance in other sports, the ACTN3 R577X polymorphism, in contrast to ACE I/D, was not significantly associated with elite swimming status when considered individually. However, the combined analysis of the two loci suggests that the co-occurrence of the ACE I and ACTN3 X alleles may be beneficial to swimmers who compete in long distance races
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