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EN
Photooxidation is a method of tissue fixation in the construction of bioprostheses. This process leads to the tissue protein crosslinking under light and dye. In the present study, the influence of methylene blue and methylene green on the effectiveness of pulmonary valve and aorta collagen modification was evaluated on the basis of photooxidized tissues sensitivity to pepsin digestion. The components of tissue hydrolysates were characterized electrophoretically. The tissues photooxidized in the presence of employing dyes showed increased resistance to enzymatic degradation in comparison to the unfixed ones. However, methylene green was more effective in valve and aorta collagen crosslinking. Hydrolysates of the tissues fixed in the presence of mentioned dye displayed decrease in collagen chains and their aggregates? content.
EN
Collagen is one of the most useful biomaterials. The medical application of collagen tissues requires specific fixation procedures. Dye-mediated photooxidation is a method of the new collagen crosslink formation. In this study, the influence of sorbitol on the extent of porcine pericardial collagen crosslinking was investigated. The collagen modification was estimated on the basis of pericardial susceptibility to pepsin digestion. The hydrolysate components were characterized electrophoretically. The significant collagen crosslinking was found in all photooxidized samples, in comparison with the untreated ones. However, the most enhanced collagen modification was observed for pericardium photooxidized with 0,5% and 0,9% sorbitol, as compared to the samples fixed without sorbitol.
EN
The use of biological materials in construction of bioprostheses requires the application of different chemical or physical procedures of fixation increasing bioprostheses resistance to enzymatic or chemical degradation and reducing their antigenicity. Methods typically concentrate on creating additional intra- and intermolecular chemical bonds between collagen molecules. This review focuses on the various methods of stabilization of collagenous tissues including chemical fixatives and physical agents.
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