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Modification of collagen film by certain chemical agents

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Lesiak-Cyganowska E., Sladowski D., Komender J.

Archivum Immunologiae et Therapiae Experimentalis

2001

vol. 49

issue 3

247-251

Morphological changes and the content of free carboxyl groups in bovine collagen (type I) film under the influence of trypsin, hydrochloric acid (HCl) and ethylenediaminetetraacetic acid (EDTA) were studied. Incubation with trypsin and HCl was found to cause some delamination of the film and the appearance of some low-density spots. Incubation with EDTA did not cause any morphological changes. A high concentration of free carboxyl groups (10-fold higher than in control) was seen after incubation with trypsin.

In vitro culture of human epithelial cells on a modified xenogenic collagen support

100%

Lesiak-Cyganowska E., Sladowski D., Jankowska E., Komender J.

Archivum Immunologiae et Therapiae Experimentalis

2001

vol. 49

issue 3

253-259

Human epithelial cells (HeLa, HaCaT, NHK) were cultured in vitro on chemically modified collagen membranes. Adhesion to the support was measured by estimation of the percentage of adhering 51Cr-labeled cells. Proliferation was estimated with the XTT test. Morphological observations of cells growing on HCl-treated collagen were performed using histological and electron microscopic techniques. HCl and trypsin-modified xenogenic collagen was found to be a good support for human cells in vitro. EDTA-incubated collagen enhanced neither adhesion nor proliferation. The best adhesion and proliferation were found on HCl-treated collagen, depending, however, on the kind of cells.

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2 Archivum Immunologiae et Therapiae Experimentalis

2 2001

2 Komender J.

2 Lesiak-Cyganowska E.

2 Sladowski D.

1 Jankowska E.

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Modification of collagen film by certain chemical agents

In vitro culture of human epithelial cells on a modified xenogenic collagen support