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1

Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig

100%
Svobodova K., Bilek K., Knoll A.
Journal of Applied Genetics
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2008
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vol. 49
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issue 3
263-265
EN
The aim of this study was to develop a set of reliable reference genes for quantification of mRNA expression in the pig. The mRNA expression stability in pig tissues was studied for 4 genes: EEF1A1, GAPDH, HPRT1 and TOP2B. The level of expression was characterized by Ct values for each gene and each tissue. By using the geNorm algorithm, the stability of the reference genes was determined in the diaphragm, heart, kidney, liver, lungs, longissimus muscle, and spleen. On the basis of this information, suitable reference genes can be selected for mRNA expression studies in relevant pig tissues.
2

The retinal fascin gene 2 (FSCN2) ? partial structural analysis and polymorphism detection in dogs with progressive retinal atrophy (PRA)

100%
Horak P., Knoll A., Dvorak J.
Journal of Applied Genetics
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2006
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vol. 47
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issue 4
361-364
EN
The retinal fascin 2 gene (FSCN2) underwent a molecular analysis, a search for polymorphisms and an evaluation as a candidate gene for retinopathies in dogs. Specific fragments of the gene encompassing partial exon 1 and intron 1, and exons 2?5 with respective introns were sequenced and these data were deposited in the GenBank database. Three distinct polymorphic sites detectable with PCR-RFLP were found ? AM050719: g.237G > A, AM050719: g.525A > G, and AM050720: g.1071A > G. No positive associations between these polymorphisms and the PRA-clinical status were observed in the investigated population consisting of Poodles, American Cocker Spaniels, and English Cocker Spaniels. In spite of that, the FSCN2 gene remains an excellent candidate gene for retinopathies in dogs and the results can contribute to further research in this field.
3

Associations of the IGF2 gene with growth and meat efficiency in Large White pigs

76%
Kolarikova O., Putnova L., Urban T., Adamek J., Knoll A., Dvorak J.
Journal of Applied Genetics
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2003
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vol. 44
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issue 4
509-513
EN
The insulin-like growth factor 2 gene (IGF2) has been described in several studies as a candidate gene for meat efficiency in pigs. IGF2 is a member of the growth factors family and has an effect on development of muscle tissue. The effect of IGF2 gene polymorphism on meat efficiency was analysed in a population of 121 Large White pigs. A PCR-based test and RFLP methods were used for detection of genotypes. Allele A, lacking the restriction site, was characterised by the presence of a 0.9-kb fragment. In allele B, the amplimer was cut into a 0.8-kb fragment and some barely detectable fragments. The statistical analysis was carried out according to the General Linear Model procedure. The genotype frequencies observed were: 1.65%, 33.88%, 64.46% for AA, AB and BB genotypes, respectively. There was a significant difference (P 0.05) between the AB and BB genotypes in live weight before the test. A significant association between AB and BB genotypes and body weight before the test was found. No significant difference in other traits of growth and meat efficiency was observed (P > 0.05).
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