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EN
This work reports the results on bioconversion of two lignocellulose wastes, i.e. wheat waste and faba bean hulls by fungi T. reesei and A. niger. The efficiency of lignocelluloses biodegradation after culturing the fungi was determined on the basis of the reduction of fibre content in the substrates, increase of protein in the preparations obtained, and the yield of protein biosynthesis. The experiments included the determination of the effect of various technological variables on the process intensification, i.e. method of culturing (shake- or surface culture), type of culture (single strains or mixed culture), raw material pre-treatment (physico-chemical or enzymatic treatment), and additional sources of carbon in the medium, inducing the biosynthesis of cellulases. In selected preparations after culturing the fungi, the changes in the composition of dietary fibre (soluble, SDF and insoluble, IDF) and simple sugars in the IDF fraction were analysed in detail.
EN
Hydrostatic extrusion is a technique which allows to produce rods with ultrafine grains and unexpectedly enhanced mechanical properties caused by grain refinement. However, the mechanism of such a refinement is not fully understood at this stage. 6xxx aluminium alloys series are usually processed by extrusion. In this study, commercial 6082 aluminium alloy was extruded at ambient temperature in a cooled die in two stages to the true strain of ε =3.2. Such a processing results in a not fully refined microstructure which allows to study different stages of grain refinement. The texture, dislocation substructures and grain refinement were investigated using electron backscatter diffraction and transmission electron microscopy techniques. The results revealed that two main texture components are present in the extruded rods - ⟨111⟩ fiber texture and ⟨001⟩ recrystallized grains. Transmission electron microscopy inspection revealed dislocation structures that can be associated with different stages of plastic deformation according to the low energy dislocation structures hypothesis proposed by Kuhlmann-Wilsdorf.
EN
Over the last few decades the use of dental implants has been increasing everyday as a solution for partial or full edentulism. The osseointegration process has to be fully completed to reach a prosthetic phase, so that patient can start to use the dental implants functionally. One of the most important factors affecting osseointegration is the surface properties of dental implants. Surface roughness of dental implant is vital to increase bone anchoring and maintain the biomechanical stability. In addition to successful application of dental implants, rapid osseointegration has also been desired as a favorable situation. It is demonstrated that there is a strong correlation between the successful application and rapid osseointegration of dental implants and the surface morphology and characteristics of dental implants. From this point of view, investigation of the surface morphology of implants has a vital importance for manufacturers. The aim of our study was to evaluate the surface topography and homogeneity of dental implants with different surface modifications by means of 2D and 3D observation methods.
EN
The ability of four strains of Lactobacillus sp. two strains of Bifidobacterium sp. and one strain of Listeria mnocytogenes to adhere to human intestinal cell lines Caco-2, HT-29 and Int 407 was examined. Well-developed monolayers of intestinal cells were obtained when initial concentration of Caco-2 cells was 1 x 104/cm2, HT-29 cells 4.2 x 104/cm2, and Int 407 cells 2 x 104/cm2. The appropriate fetal bovine serum additions for Caco-2, HT-29 and Int 407 were 20%, 10% and 10%, respectively. The reduction of serum addition decreased intestinal cell density and prolonged monolayer development. The highest cell densities in epithelial monolayer were obtained in the Int 407 cell cultures. The yield of bacterial adhesion was strain ? dependent. Significant differences were also observed in bacteria adhesion to individual intestinal cell lines. The best adhesion ability to Caco-2 exhibited Lactobacillus rhamnosus GG and Bifidobacterium bifidum. The highest adhesion to HT-29 line demonstrated B. bifidum and Lactobacillus acidophilus LC1. The adhesion of bacteria to Int 407 was much lower. Significant effect on bacteria adhesion has their cell density being in contact with intestinal monolayer. The adherence of Listeria monocytogenes to Caco-2 and HT-29 was very low in the range of 0.2% and 6.0%, respectively.
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