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1

Identification of wheat cultivars using molecular methods

100%
Borsuk P., Moraczewski I., Czaplicki A.
Biotechnologia
|
2001
|
issue 1
119-126
EN
We used RAPD created polymorphism to analyze the diversity of wheat cultivars. Preliminary analysis of the wheat genome polymorphism by the RAPD indicated that the technique is useful for the identification of similarities between cultivars. Using 10 different primers we studied the pattern of specific amplified DNA fragments ? bands of 90 wheat varieties ? and we found that three of these primers generated high level of polymorphism. One of these primers, selected for further studies, generated 38 bands with molecular weight in the range from 215 to 1300 bp. For the purpose of diversity analysis, all polymorphic loci were scored as present/absent. The bivariate 1-0 data were used as the raw matrix. A square symmetric matrix of similarity was obtained using the Czekanowski-Sorensen coefficient. Similarity matrix was then used for cluster analysis using the Unweighted Pair Group Method of Averages (UPGMA) technique. A result was a fingerprint of all varieties, that allows for their identification. Using numerical taxonomy we identified in the analyzed group of varieties 10 clusters that indicated high variability of genetic material in this group.
2

Polish transgenic cereal crops

88%
Zimny J., Sowa S., Menke-Milczarek I., Czaplicki A., Sowa A.
Biotechnologia
|
2000
|
issue 4
80-87
EN
First transgenic cereal plants have been obtained in Poland seven years ago. Within the time other cereals like wheat, rye and barley have been also transformed. The prerequisite for that was a very efficient regeneration system by somatic embryogenesis. Generally the basic study on transgenic cereals are quite advanced but the question is how to include transgenic lines in to practical breeding process? Most of genes, promoters and transformation methods are patented and probably Polish breeders will never afford to buy the licences. Though there is a need to concentrate the future work in Polish institutes on identification and isolation of genes of interest. Than to transfer them to plants and register transgenic varieties. According to the Polish law it is allowed to carry out the field experiments, but it is not possible to register the plant variety.
3

Consequences of transformation of embryogenic Triticale cell

88%
Zimny J., Czaplicki A., Menke-Milczarek I., Oleszczuk S., Sowa S.
Biotechnologia
|
2000
|
issue 2
64-72
EN
Plants carrying foreign genes have been obtained for many crops including wheat, rice, maize, barley and Triticale. The most important aspect for practical breeding is the regeneration of whole plants from a specific cell possessing the desired agronomic properties. Particle bombardment provided the necessary breakthrough for the efficient transformation of cereals. Efficient regeneration is a prerequisite for all transformation techniques. The aim of the presented work was to study the progeny of transgenic plants of the allohexaploid cereal species Triticale. By combining an efficient regeneration system with the successful particle bombardment method we were able to obtain transgenic Triticale plants. Transgene expression was sometimes unstable and generally resulted in the decline of the expression, although some lines showing stable expression were also selected. In our laboratory several generations of androgenic doublehaploid transgenic lines have been regenerated and multiplicated. The integrated transgenes were detected in Triticale lines by in situ hybridisation method. The stability of trangenes has been studied on ten generations. A regeneration system from single cell to plant combined with microprojectile bombardment appeared to be the most efficient transformation method for Triticale. Numerous chimeric genes are now available for research. Some of these genes may appear useful in the future breeding of Triticale.
4

Genetic relationship between Polish and Chinese strains of the mushroom Agaricus bisporus (Lange) Sing., determined by the RAPD method

76%
Staniaszek M., Marczewski W., Szudyga K., Maszkiewicz J., Czaplicki A., Gian G.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 1
43-47
EN
The genetic relationship between twenty-six strains of Agaricus bisporus were analysed by the RAPD (random amplified polymorphic DNA) method. DNA amplification was performed with the use of twelve arbitrary 10-mer primers. Four primers, which gave polymorphic band patterns were chosen for RAPD analysis. In total, they gave 24 distinguishable bands, of which nine were polymorphic. The conducted research showed that there is a great genetic similarity among the examined strains. Low polymorphism of the strains may be a proof of a limited genetic pool used in the cultivation of those strains.
5
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Strategies influencing energy efficiency of lighting solutions

72%
Pracki P., Wiśniewski A., Czyżewski D., Krupiński R., Skarżyński K., Wesołowski M., Czaplicki A., Pracki P., Wiśniewski A., Czyżewski D., Krupiński R., Skarżyński K., Wesołowski M., Czaplicki A.
|
2020
|
issue 4
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