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1

Application of microsatellities in genetic mapping of quantitative trait loci in farm animals

100%
Korwin-Kossakowska A.
Biotechnologia
|
1996
|
issue 2
178-183
EN
Microsatellites are tandem repeats of a simple sequence that occur abundantly and at random throughout most eukaryotic genomes.Mammalian microsatellite loci have been shown to be highly polymorphic due to variation in the number of repeat units.They are very good markers for quantitative traits loci (QTLs) mapping studies.Swedish Group from Agricultural University in Uppsala found evidence for QTLs on chromosome 4 with large effects on growth , fat deposition and length of the small intestine based on the analysis of genetic linkage between quantitative level and 105 genetic markers (68 microsatellities loci and 37 others markers).The localization of the gene affecting the ovulation rate and litter size on chromosome 6 in sheep has been documented on the basis of identification of genetic linkage between Booroola fecundity (FecB) gene and two microsatellite loci (OarHH55 and OarAE101) by a New Zealand scientist.Out of all types of actually known genetic markers microsatellites are most useful for QTLs mapping.
2

Radiation Hybrid Panel--modern method for physical mapping of porcine genome

63%
Korwin-Kossakowska A., Kuryl J.
Biotechnologia
|
2004
|
issue 3
184-192
EN
The creation of high-resolution maps is central to genetics: maps allow genes to be associated with phenotypes and can be used as tools for gene cloning. Maps for the human genome and other mammalian species are being created both by classical recombination methods and by physical mapping techniques. One powerful method for creating physical maps is the use of irradiation and fusion gene transfer (IFGT) to make radiation hybrid maps. Goss and Harris who showed that chromosome fragments, generated by lethal irradiation of donor human cells, could be 'rescued' by fusion to rodent recipient cells originally developed this technology. Each resulting hybrid cell, which includes both hamster and human DNA, can be grown up to yield a hybrid cells or hybrid cell line of cells containing the same random subset of the human genome. A radiation hybrid panel (RH) consists of a number of different hybrid cell lines (sometimes just called hybrids or clones). The recombinant clone is tested for the presence or absence of each DNA marker (for example microsatellites). By estimating the frequency of breakage, and thus the distance between markers, it is possible to determine their order along chromosome. The successful use of RH panels to map the human genome has led to the development of WG-RH (whole genome radiation hybrid) maps in other species, for example of the porcine genome. This map provides a resource for rapid, large scale physical mapping of the swine genome and facilitates resolving genetic and physical distances prior to designing strategies for positional candidate cloning of the gene(s) contributing to economically important traits. WG-RH panels have now been used to create chromosomal maps in several species: rat, horse, bovine, cat, dog and mouse.
3

Polymorphism of the porcine growth hormone gene and its linkage to hormone gene and its linkage to microsatellites S0083 and S0090

39%
Korwin-Kossakowska A., Pierzchala M., Kuryl J., Zwierzchowski L., Cymerowska-Prokopczyk I., SiadkowskaE.
Journal of Applied Genetics
|
1999
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vol. 40
|
issue 2
85-91
EN
Polymorphism of microsatellites S0083 and S0090 as well as of the second exon and second intron of the pGH (porcine growth hormone) gene was determined for 293 pedigrees obtained from crossing of 12 F1 (Zlotnicka Spotted x Polish Large White) boars with 64 F1 (Zlotnicka Spotted x Polish Large White) sows, experimental material arranged for a QTL mapping project. Microsatellites were genotyped by capillary electrophoresis of PCR products in an ABI PRISM 310 Genetic Analyser PERKIN-ELMER. The PCR-RFLP polymorphism of the GH gene was identified using HaeII and MspI restriction endonucleases. The following order of linked loci was established: GH-S0083-S0090. This is important information for the mapping of QTLs on chromosome 12.
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