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1
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The effectivity of metabolite secondary extract of bacteria associated with sea grass (Cymodocea rotundata) for Vibriosis treatment in tiger shrimp (Penaeus monodon)

100%
Lili W., Maulana G., Astuty S., Rosidah R.
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vol. 26
96-115
EN
The natural occurrence of the shrimp (Penaeus monodon Fabricius, 1798) and seagrass (Cymodocea rotundata Asch. & Schweinf.) is found in the area of Indian Ocean, Indonesia, for central Pacific. Vibriosis can cause death in larvae, juvenile and adult shrimp almost close to 100%. Antibacterial substances from secondary metabolites are produced by plants and animals to reduce the use of synthetic antibiotics. The purpose of this research was to determine the ability of secondary metabolites contained in the bacterial extract of the association of sea grass Cymodocea rotundata (isolate code BA.1) to treat vibriosis in tiger shrimp (Penaeus monodon). This research was conducted at the Laboratory of Integrated Biotechnology and Laboratory of Aquaculture, Fisheries and Marine Sciences Faculty, Universitas Padjadajaran, and at the Brackish and Southern Ocean Aquaculture Development Center Pangandaran, from June to September 2018. The research method used was experimental, with completely randomized design (CRD) consisting of five treatments with three replications including; control treatment (concentration of 0 mg·L–1), 150 mg.L–1, 300 mg·L–1, 450 mg·L–1 and 600 mg·L–1. Observations made include clinical symptoms (morphology, behavior) and survival rate. Clinical symptoms of infected tiger shrimp during the in vivo test were red spots on the abdomen and necrosis of some leg and tail segments. The behavior of tiger shrimp mostly shows less responsive movements to fish-feed on the first day, and showing responsive and active behavior on the fifth day. In vivo test results for 14 days showed that the highest survival rate of 51.67% was in case of the addition of antibacterial extracts of BA.1 with a concentration of 300 mg·L–1.
2
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Effect of Types Isolated Lactic Acid Bacteria on Hematocrit and Differential Leukocytes Fingerling Common Carp (Cyprinus carpio L.) Infected with Aeromonas hydrophila bacteria

100%
Shabirah A., Rosidah R., Mulyani Y., Lili W.
World News of Natural Sciences
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2019
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vol. 24
22-35
EN
This study aims to see the condition of hematocrit levels and differential leukosit after immersion in LAB isolated and after in vivo, using Aeromonas hydrophila bacterium. LAB isolated were obtained from the organs of the common carp intestine. The study used a Completely Randomized Design (RAL) consisting of four treatments and three replications. The treatment used was immersion of test fish for 24 hours in several isolates of LAB with a density of 108, namely A: without LAB isolate (control), B: isolate CcB7, C: isolate CcB8, D: isolate CcB15. Immersion was done three times, with a frequency of seven days. Parameters observed included hematocrit levels, leukocyte differentiation and survival rate. The results showed that immersion in LAB isolate CcB15 will enhance the differential leukocytes and hematocrit levels that serve as indicators of carp body resistance, which is shown by high levels of white and red blood cells.
3
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Utilization of Liquid Commercial Probiotics to Improve Survival and Growth of Siamese Catfish Fingerlings (Hypopthalmus pangasionodon (Sauvage, 1878))

100%
Meidi W. S., Lili W., Iskandar I., Suryadi I. B. B.
World News of Natural Sciences
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2019
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vol. 24
54-63
EN
The purpose of this research is to determine the effect of liquid commercial probiotics usage on improving the survival rate and growth of Siamese catfish fingerlings. This research was conducted on March to May 2018, at Building 4 Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. This study used an experimental method with Completely Randomized Design (CRD), which consisted of four treatments and three replications. The treatments are feed without the addition of probiotics, and feed with additional probiotics at doses of 2, 4, and 6 ml / kg of feed. The parameters observed were survival rate, absolute weight, absolute length, feed conversion ratio, proximate test analysis and water quality. Data on absolute weight, absolute length, feed conversion ratio and survival rate were analyzed using analysis of variance, while nutrition changes of fish feed and water quality were analyzed descriptively. The results showed that the best addition of probiotics for feed, is with probiotic doses of 2 ml / kg of feed, which resulted in a 100% survival rate, an absolute weight value of 1.03% and a feed conversion ratio of 1.13.
4
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The Effect of Addition Marigold-Meal to Artificial Feeds for Increasing Color Intensity of Koi Fish (Cyprinus carpio Linnaeus, 1758) Strain Kohaku

100%
Lili W., Iskandar I., Rhamdhan R. M., Grandiosa R.
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EN
Feed greatly affects the growth and health of fish, however it also functions to increase the value of the color intensity when specific additional ingredients are added. One of the important ingredients, marigold-meal is one of the natural carotenoid sources. This research aims to determine the optimum concentration of marigold-meal in the artificial feed to increase the intensity color of koi fish, specifically the kohaku strain. The method used was an experimental Completely Randomized Design, consisting of five treatments and four replications. Parameters observed were color intensities analyzed using Kruskal-Wallis analysis, if there were significant differences, Z test would be performed. Research results were the color intensity enhancement of koi fish tails and head until the 40th day showed the highest value in the treatment concentration of 0.0% marigold-meal (positive control) with an average score of 8.00 and 10.00. This value was not different from the treatment concentration of 2.0% marigold-meal and concentration 1.5% marigold-meal. However, this value was not significantly different from concentration of 1.5% marigold-meal. The lowest average score on the tail and head was treatment concentration of 0.0% marigold-meal (negative control). The optimum concentration of the addition of marigold-meal resulting the best color intensity increase was in 1.5% concentration of marigold-meal with the color intensity value of 5.97 in the tail and 6.50 in the head of koi fish Kohaku strain.
5
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Effect of Adding Pumpkin Flour and Carrot Flour on Changes in Color Intensity of Goldfish (Carassius auratus L.) Strain Oranda

88%
Ahlam R. N., Lili W., Hamdani H., Rosidah R., Bangkit I.
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vol. 26
52-60
EN
Goldfish is one of the most famous ornamental fish because of its body shape and color which was interesting to be maintained. The purpose of this research was to find the optimum dose of pumpkin and carrot flour combination on artificial feed to increase color brightness of goldfish. Complete Randomized Design with four treatments and four replications were used in the experiment. For the treatments, pumpkin flour 15%, pumpkin flour 7.5% with carrot flour 2.5% and carrot flour 5%, were studied. The parameters observed were color intensity, survival rate, and water quality. The results showed that supplementation of combination of pumpkin flour 7.5% with carrot flour 2.5% gave the highest color intensity of goldfish with TCF code 0815 and score 2.11 with 100% survival rate. Water qualities during the research were still in optimal condition.
6
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Total phenolic, flavonoid content and antioxidant capacity of stem bark, root, and leaves methanolic extract of Rhizophora mucronata Lam.

88%
Mulyani Y., Syaputra N. D., Dewi K. C., Lili W., Agung M. U. K.
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vol. 26
118-127
EN
The study was carried out to evaluate the phytochemical constituents, antioxidant activity and the content of total phenolic and flavonoid in the methanolic extract from stem bark, root, and leaves of Rhizophora mucronata from Karangsong, Indramayu Regency, West Java, Indonesia. The antioxidant activity was evaluated using 2,2-diphenyl-1-picrilhydrazyl (DPPH) assay. Total phenolic and flavonoid contents were determined according respectively to Folin-Ciocalteau method, and aluminium trichloride method. The methanol extract from stem bark of R. mucronata contained secondary metabolite such as phenolic, flavonoid, tannin and saponin. The stem bark of R. mucronata showed the highest phenolic and flavonoid content and possessed a higher antioxidant activity (IC50 84.80 µg·m/L) than that of root (IC50 166.95 µg·m/L) and leaves (IC50 90.51 µgmL1). Total phenolic and flavonoid content in the stem bark of R. mucronata were 131.91 ±1.7 mg gallic acid equivalents/g and 902 ±0.7 mg quercetin equivalents/g, respectively.
7
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The Effect of Probiotic Addition in Commercial Feed to Growth and Survival Rate of Sangkuriang Catfishes (Clarias gariepinus (Burchell, 1822))

88%
Lili W., Hartanto R., Kurniawati N., Herawati T., Bangkit B. S. I.
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vol. 21
130-140
EN
This study was conducted to determine the effect of probiotic addition in commercial feed to the growth and survival rate of Sangkuriang catfish (Clarias gariepinus). This research was conducted at the Ciparanje Experimental Pond Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Jatinangor, West Java. The method in used this research was Completely Randomized Design with four treatments and four replications. The treatment was the variances of probiotic addition which consisted of control (without probiotics), probiotic addition at as much as 0.5 grams / kg of feed, 1 gram / kg of feed and 1.5 grams / kg of feed. The parameters observed were specific growth rate (SGR), absolute biomass, survival rate (SR) and feed conversion ratio (FCR). Data were analyzed using Variant Analysis, at 95% confidence level, and continued with Duncan's Multiple Range Test. The results showed that the increase in the daily growth rate of Sangkuriang catfish was straight-line with the addition of probiotics. Furthermore, commercial probiotics with a dose of 1 gram / kg of feed resulted in specific growth rate of 4.22%, absolute weight of 11 grams, survival rate (SR) of 97.5% and FCR of 0.89.
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