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Crystal and molecular structure of hexagonal form of lipase B from Candida antarctica

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Strzelczyk P., Bujacz G., Kiełbasiński P., Błaszczyk J.

2016

vol. 63

issue 1

103-109

During crystallization screenings of commercially available hydrolytic enzymes, the new, hexagonal crystal form of CAL-B, has been discovered and hereby reported. The NAG molecules, which were closing the glycosylation site in the orthorhombic form, in hexagonal structure make the glycosylation site open. It is unknown whether the opening and closing of the glycosylation site by the 'lid' NAG molecules, could be related to the opening and closing of the active center of the enzyme upon substrate binding and product release.

High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor

88%

Brzezinski K., Stepkowski T., Panjikar S., Bujacz G., Jaskolski M.

2007

vol. 54

issue 3

537-549

The fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes α1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain. Mutational data combined with structural and sequence alignments indicate residues of potential importance in GDP-fucose binding or in the catalytic mechanism. They are all clustered in three conserved sequence motifs located in the C-terminal domain.

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2 Acta Biochimica Polonica

1 2016

1 2007

2 Bujacz G.

1 Brzezinski K.

1 Błaszczyk J.

1 Jaskolski M.

1 Kiełbasiński P.

1 Panjikar S.

1 Stepkowski T.

1 Strzelczyk P.

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Crystal and molecular structure of hexagonal form of lipase B from Candida antarctica

High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor