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1

Characterization and serological classification of a collection of Proteus penneri clinical strains

100%

Drzewiecka D., Zych K., Sidorczyk Z.

Archivum Immunologiae et Therapiae Experimentalis

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2004

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vol. 52

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issue 2

121-128

EN

Chronic Granulomatous disease bacteria of the genus Proteus, which are a common cause of urinary tract infections, are divided into four species: P. mirabilis, P. vulgaris, P. penneri, and P. hauseri, and three unnamed genomospecies, Proteus 4, 5, and 6 (single-strain species P. myxofaciens was isolated from the gypsy moth). Establishing the serological classification of these species would aid in completing the classification scheme of the whole genus Proteus and in applying serological methods in diagnostic procedures and epidemiological investigations for these opportunistic pathogens. The aim of this research was a serological characterization and classification of 57 Proteus penneri clinical strains, isolated from patients from different countries all over the world, into Proteus O serogroups. Purified lipopolysaccharides (LPSs) extracted from 57 P. penneri strains were used as antiandgens in enzyme immunosorbent assay (EIA), SDS/PAGE, and Western blot techniques, and alkali-treated LPSs in passive immunohemolysis test (PIH), inhibition of PIH, and absorption of rabbit polyclonal O-antisera. Results: That result confirms the serological distinction of this species within the genus Proteus, and may have diagnostic significance. Conclusions: As a result of serological studies of LPSs extracted from the P. penneri strains, one new Proteus serogroup, represented by the P. penneri 97 strain, was established. Three further strains were classified into the Proteus serogroup O8, which had not contained any P. penneri strains before. All the remaining strains were classified into 11 already existing Proteus O serogroups. It is important to emphasize that 72% of studied strains were classified into serogroups that contain P. penneri strains only.

2

Serological classification and epitope specificity of Proteus vulgaris TG 251 from Proteus serogroup O65

76%

Zych K., Kolodziejska K., Drzewiecka D., Perepelov A. V., Knirel Y. A., Sidorczyk Z.

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2007

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vol. 55

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issue 3

187-191

EN

Introduction: Proteus rods are currently subdivided into five named species, i.e. Proteus mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens, and three unnamed Proteus genomospecies 4 to 6. Based on the serospecificity of the lipopolysaccharide (LPS; O-antigen), strains of P. mirabilis and P. vulgaris were divided into 49 O-serogroups and 11 additional O-serogroups were proposed later. About 15 further O-serogroups have been proposed for the third medically important species, P. penneri. Here the serological classification of P. vulgaris strain TG 251, which does not belong to these serogroups, is reported. Serological investigations also allowed characterization of the epitope specificity of its LPS. Materials and Methods: Purified LPSs from five Proteus strains were used as antigens in enzyme immunosorbent assay (EIA), SDS/PAGE, and Western blot and alkali-treated LPSs in the passive immunohemolysis (PIH) test, inhibition of PIH and EIA, and absorption of the rabbit polyclonal O-antisera with the respective LPS. Results: The serological studies of P. vulgaris TG 251 LPS indicated the identity of its O-polysaccharide with that of P. penneri O65. The antibody specificities of P. vulgaris TG 251 and P. penneri O65 O-antisera, were described. Conclusions: P. vulgaris TG 251 was classified to the Proteus O65 serogroup. Two disaccharide-associated epitopes present in P. vulgaris TG 251 and P. penneri O65 LPSs are suggested to be responsible for cross-reactions with three heterologous Proteus strains.

3

Structural and serological characterization of the lipopolysaccharide from proteus penneri 20 and classification of cross-reacting proteus penneri strains 10, 16, 18, 20, 32 and 45 in proteus serogroup O17

64%

Sidorczyk Z., Toukach F. V., Zych K., Arbatsky N. P., Drzewiecka D., Ziolkowski A., Shashkov A. S., Knirel Y. A.

Archivum Immunologiae et Therapiae Experimentalis

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2002

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vol. 50

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issue 5

345-355

EN

O-specific polysaccharide (O-antigen) of the lipopolysaccharide of Proteus penneri 20 was studied using sugar analysis along with various one- and two-dimensional NMR spectroscopy techniques. The structure of the polysaccharide was established. It has the same carbohydrate backbone structure as that described earlier for P. penneri 16, in which the positions of the O-acetyl groups have not been determined. P. penneri 20 O-antiserum showed a strong cross-reactivity with the lipopolysaccharides of P. penneri 10, 16, 18, 32, 45 and P. mirabilis O17. These data enable classifying these strains together with P. penneri 20 in one Proteus serogroup, O17.

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Characterization and serological classification of a collection of Proteus penneri clinical strains

Serological classification and epitope specificity of Proteus vulgaris TG 251 from Proteus serogroup O65

Structural and serological characterization of the lipopolysaccharide from proteus penneri 20 and classification of cross-reacting proteus penneri strains 10, 16, 18, 20, 32 and 45 in proteus serogroup O17