Search results

1

The average time complexity of probabilistic algorithms for finding generators in finite cyclic groups

100%

Adamski T., Nowakowski W.

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2015

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issue 4

2

Security of Nyberg-Rueppel digital signatures without message recovery

100%

Adamski T., Nowakowski W.

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2014

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issue 4

3

Barley haploid from reciprocal crosses of Hordeum bulbosum (2x) and H.Vulgare (2x)

100%

Surma M., Adamski T.

Journal of Applied Genetics

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1995

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vol. 36

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issue 1

11-16

EN

Results of crosses between Hordeum bulbosum (2x) and H.vulgare (2x) are presented.Nine H.vulgare genotypes of different crooability with H.bulbosum were treated both as female and male parents Immature embryos were cultured in vitro according to Adamski and Surma (1994).Seeds set, embryo development and obtained haploid plants were recoded.It was shown that seed setting and haploid plant efficiency (haploids/100 florets) were about ten times lower in H.bulbosum x H.vulgare than H.vulgare x H.bulbosum.The cvs.Vada, Apex and the doubled haploid line KA7/3 (related to Apex) which showed a low crossability in H.vulgare x H.bulbosum,gave a relatively high seed setting in reciprocal crosses.The obtained results indicte that partial incompatibility of some H.vulgare genotypes with H.bulbosum could be cytoplasmatically influenced.

4

The influence of genotypes on production of barley haploids with Hordeum bulbosum cytoplasm

100%

Adamski T., Surma M.

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EN

Hordeum bulbosum (2x) was crossed to H.vulgare (2x) to estimate the influence of genotypes on production of barley haploids with H.bulbosum cytoplasm.Four H.bulbosum clones were pollinated with 11 H.vulgare cultivars and doubed haplod lines.Immature embryos were cultured on the B5 medium (Gamborg et al.1968).Seed setting, embryo differentiation, haploid plant development and haploid plant efficiency were scored and computed using multivariate analysis of variance as well as cluster and canonical variable analysis.Only the influence of H.vulgare genotypes on haploid production appeared to be significant.Haploid plant efficiency was very low (average 1.45%) and depended mainly on seed setting.Obtained barley haploids and consequently, doubled haploid lines with alien cytoplasm can constitute precise material for genetic studies dealing with cytoplasmic inheritance.

5

The use of laser light for obtaining barley haploids by Hordeum bulbosum method

81%

Rybinski W., Surma M., Adamski T.

Biotechnologia

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2001

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issue 1

143-147

EN

The effect of helium-neon laser with wavelength of 632 nm on seed setting in a cross of Hordeum vulgare x H. bulbosum was studied. The seeds before sowing as well as immature embryos were irradiated with laser light. Material not irradiated with laser beams constituted the control. It was shown that stimulative dose of laser beams increased the number of seeds/100 pollinated florets in comparison with the control combination. After laser treatment, the seed setting of the line HG156, and cultivars Vada and Apex was higher by 13.0, 7.9 and 3.2%, respectively. The number of obtained haploids/100 cultured embryos irradiated with laser light was also higher in comparison with the control by 1.8, 0.9 and 10.8%, respectively. The obtained results show that treatment with laser beams was more effective at the first step of haploid production (seeds/100 florets) than at the next step (haploids/100 embryos cultured). From the practical point of view, better results can be obtained by irradiation of seeds before sowing than by irradiation of immature embryos. Cultivar Apex with a positive reaction on irradiation of embryos was an exception here.

6

A simple test for detection of linkage

81%

Surma M., Kaczmarek Z., Adamski T., Kaczmarek.

Journal of Applied Genetics

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2002

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vol. 43

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issue 2

171-174

EN

The paper presents a proposition for detection of linkage of genes responsible for metrical traits. Taking into account the expected means for early generations (F1, F2, F3) and a population of homozygous lines (in this case doubled haploid lines, DH derived from a cross between two homozygous parents) as well as estimators of genetic parameters m, [d], [i], [h] and [l], the expected values for these parameters in the presence of linkage have been formulated. It was found that when there is no linkage, the expression F1 ? 6F2 + 8F3 ? 3DHmean is equal to zero. Thus, an experiment covering DH lines and F1, F2, F3 hybrids makes it possible to obtain, beside information of interest, also information on presence or absence of linkage.

7

Theoretical bases for detection of linkege of genes between two quantitative characters in the presence of nonallelic interaction

81%

Kaczmarek Z., Surma M., Adamski T.

Genetica Polonica

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1994

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vol. 35

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issue 1-2

53-62

8

Methods to predict transgressive segregation in barley and other self-pollinated crops

81%

Kuczynska A., Surma M., Adamski T.

Journal of Applied Genetics

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2007

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vol. 48

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issue 4

321-328

EN

Most of agronomically important characters are biometric traits. An improvement of these traits in cultivated plants by deriving segregants superior to parents, which could be developed as cultivars, is a main goal in breeding of self-pollinated crops. Two problems need to be solved: when will the progeny be better than its parents and how can a genetic potential of a given pair of parental genotypes be predicted? In this paper, transgressive segregation in homozygous barley populations is shortly reviewed. Various approaches to choosing parental forms are shown, and a theoretical method for predicting the frequency of transgressive segregants in a homozygous population is presented. Additionally, relationships between parental diversity estimated with molecular markers and the progeny performance are discussed. Although the prediction of transgressive segregation is still a problem, it seems promising to apply an approach measuring the performance of the parental genotypes and estimating their genetic distance by molecular markers.

9

Numerical method for detection of linkage between genes for two metrical traits

81%

Kaczmarek Z., Surma M., Adamski T., Czajka S.

Journal of Applied Genetics

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2004

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vol. 45

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issue 1

27-35

EN

A homozygous population derived from hybrids between two homozygous parents may be used for genetic analysis of metrical traits. The paper describes the use of doubled haploids (DH) and single seed descent (SSD) lines for detection of linkage between genes conditioning two quantitative traits. A computational algorithm is presented, which facilitates matching various variants of relations between variances, covariances and means of DH and SSD populations so as to make it possible to conclude on the presence/absence of linkage. The suggested methodology is illustrated with an example concerning three quantitative traits of barley: length of the third internode, stem wall thickness, and 1000-grain weight.

10

Frequency of transgression and gene distribution in barley doubled haploid populations from first and second cycle hybrids

81%

Surma M., Adamski T., Kaczmarek Z., Kapala A.

Journal of Applied Genetics

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1998

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vol. 39

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issue 3

237-247

EN

Barley doubled haploids (DH) derived from first and second cycle hybrids were investigated in field experiments. Parental lines designed for the second cycle hybrids were three doubled haploids from the first cycle hybrids, which were observed to have the highest grain yield. Yield structure characters, crude protein content and protein fractions were analysed. Phenotypic and genetic variability and the frequency of transgression in the studied populations were calculated. For the studied traits additive, [d], and epistatic [i], effects as well as coefficient of gene dispersion were estimated. It was found that the phenotypic and genetic variability of DH populations derived from second cycle hybrids was higher than that of the original population for all the studied traits except grain yield. A greater proportion of transgressive lines than in the original population was also observed in populations from second cycle hybrids. Only one DH line exceeding the high yielding parent was found among 141 lines under study. The relationship between the frequency of transgressions and gene dispersion was recorded: the greatest number of transgressive lines occurred in those traits for which the dispersion was observed.

11

Genetic diversity among cultivars of spring barley revealed by random amplified polymorphic DNA (RAPD)

71%

Kuczynska A., Milczarski P., Surma M., Masojc P., Adamski T.

Journal of Applied Genetics

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2001

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vol. 42

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issue 1

43-48

EN

RAPD (random amplified polymorphic DNA) polymorphism was studied in 23 malting and non-malting spring barley cultivars included in the official list of Polish cultivated varieties. Twenty-four 10-mer primers were tested in each cultivar, giving altogether 149 amplification products, 45% of which were polymorphic. The number of polymorphic bands revealed by one primer ranged from 1 to 6, with an average of 2.8. Genetic distance for all pairs of compared varieties was estimated and a dendrogram was constructed using unweighted pair group method of arithmetic means. The genetic distance between cultivars ranged from 0.11 for cvs. Apex and Bryl to 0.62 for cvs. Orthega and Madonna. Of the seven malting cultivars only two (Brenda and Stratus) formed one group at D = 0.25. The genetic distance between cvs. Brenda and Scarlett, especially recommended for brewery, was equal to 0.34. The detected polymorphism appeared to be sufficient for assessing genetic distances between cultivars, but on the basis of this polymorphism groups of malting and non-malting cultivars were not clearly distinguished.

12

Assessment of RAPD markers for barley doubled haploid lines resistant and susceptible to Fusarium culmorum at seedling and adult plant growth stages

62%

Bocianowski J., Chelkowski J., Kuczynska A., Wisniewska H., Surma M., Adamski T.

Journal of Applied Genetics

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2003

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vol. 44

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issue 3

355-360

EN

Thirty doubled haploid (DH) lines of barley derived from F1 of a cross between the six-rowed cultivar Pomo and two-rowed cultivar Maresi were examined for susceptibility to Fusarium seedling blight (SB) and head blight (FHB), measured by mycotoxin (nivalenol) content of kernels. RAPD (random amplified polymorphic DNA) polymorphism was analysed by using 53 decamer primers. Amplification products (APs) were 200 bp up to 2000 bp in size on average 5.7 per primer and the total number of APs was 284, 51.06% of which were polymorphic. Only 32 APs differentiated the examined DH lines ? 19 APs for nivalenol content of kernels and 13 for seedling resistance. DH lines segregated with continuous distribution of resistance to FHB and SB. At the seedling stage all DH lines exhibited lower susceptibility than parental cultivars, but in the adult stage only two lines (MP 2 and MP 7) appeared to be more resistant to FHB, i.e. accumulated in kernels a lower amount of mycotoxin than cultivars Maresi and Pomo.

13

Yield reduction and mycotoxin accumulation in barley doubled haploids inoculated with Fusarium culmorum (W.G.Sm.) Sacc.

62%

Adamski T., Chelkowski J., Golinski P., Kaczmarek Z., Perkowski J., Surma M., Wisniewska H.

Journal of Applied Genetics

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1999

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vol. 40

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issue 2

73-84

EN

Barley doubled haploids (DH) were examined for their susceptibility to Fusarium head blight caused by Fusarium culmorum. DH lines were derived from F1 Maresi (two-rowed) ? Pomo (six-rowed) hybrids by the 'H. bulbosum' method. Doubled haploids, parental cultivars and F1 and F2 hybrids were inoculated with Fusarium culmorum (W.G.Sm.) Sacc., isolate KF350 under field conditions. The kernel infection score, number of kernels per ear, kernel weight per ear, 1000-kernel weight, and kernel fractions were recorded in inoculated and control plants. Samples of kernels were analysed for presence of nivalenol and deoxynivalenol. In the inoculated plants a reduction of kernel number, kernel weight per ear, 1000-kernel weight and percentage of plump kernels was observed. Generally, inoculation caused a significant decrease in the kernel fraction > 2.5 mm, and increase in the fractions 2.5-2.2 and < 2.2 mm. This tendency was more visible in 2-rowed than in 6-rowed lines. The nivalenol content of inoculated doubled haploids ranged from 0.16 to 7.61 mg/kg, whereas their deoxynivalenol content ranged from 0.000 to 0.253 mg/kg. Significant relationships between the kernel infection score and nivalenol content, kernel yield per ear, 1000-kernel weight and kernel fraction > 2.5 mm were observed. Transgression effects were noted in some DH lines, in which the reduction of kernel characters was lower than in parental cultivars. Doubled haploids with a positive and negative transgression for nivalenol and deoxynivalenol content were also recorded.

14

Interaction of gene effects with environments for malting quality of barley doubled haploids

52%

Kaczmarek Z., Surma M., Adamski T., Jezowski S., Madajewski R., Krystkowiak K., Kuczynska A., KaczmarekZ.

Journal of Applied Genetics

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2002

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vol. 43

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issue 1

33-42

EN

Barley doubled haploids covering a wide range of malting quality, along with their parental cultivars and F2, F3 hybrids, were investigated in six environments (three locations, two years) to study the genotype-environment (G ? E) interaction structure and the influence of environments on additive, dominance and epistatic gene effects. Grain and malt characters, such as 1000-grain weight, percentage of plump kernels, malt extract yield, protein content, Kolbach index and malt fine-coarse difference (FCD), were measured. Main effects for genetic parameters were estimated and regression analysis was used to explain the interaction of gene effects with environments. The results show that additive effects had the greatest interaction with environments for all the analysed traits, but only for malt characters this interaction was linear. Interaction of dominance effects was much lower and only in the case of 1000-grain weight, protein content and Kolbach index it proved to be significant. The results suggest that effects of heterozygous loci are more stable in contrasting environments than effects of homozygous loci.

15

Genetic determination of variability of barley doubled haploids inoculated with Fusarium culmorum (W.G.Sm.) Sacc. with regard to mycotoxin accumulation and reduction in yield traits

42%

Surma M., Adamski A. T., Adamski T., Chelkowski C. J., Chelkowski J., Golinski G. P., Golinski P., Kaczmarek K. Z., Kaczmarek Z., Kostecki K. M., Kostecki M., Perkowski P. J., Perkowski J., Wisniewska W. H., Wisniewska H.

Journal of Applied Genetics

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2000

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vol. 41

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issue 4

237-246

EN

The genetic determination of variability of barley doubled haploid (DH) lines in regard of their susceptibility to Fusarium head blight caused by Fusarium culmorum was studied. The susceptibility was evaluated in a 3-year field experiment on the basis of reduction in yield traits and mycotoxin accumulation in infected kernels. The following traits were analysed in inoculated and control plants: kernel number and weight per ear, 1000-kernel weight, percentage of plump kernels (>2.5 mm), deoxynivalenol (DON) content and nivalenol (NIV) content of kernels. On the basis of the obtained data, heritability coefficient (ratio of genotypic to phenotypic variance) was assesed, and genetic parameters as well as the number of effective factors were estimated. Heritability coefficients calculated from two-way analysis of variance, i.e. regarding the influence of years and year ? genotype interaction, appeared to be exceptionally low and ranged from 5.2% for the reduction in plump kernels to 38.2% for the reduction in 1000-kernel weight. In the case of mycotoxin accumulation about 60% of the observed variability in NIV concentration and 30% in DON concentration resulted from genetic differences among lines. Additive effects of genes were important for all the analysed traits. Significant effects of dominance and dominance ? dominance were observed for 1000-kernel weight and percentage of plump kernels. Moreover, it was found that the observed variability in yield trait reduction resulted from the segregation of 5-6 effective factors, DON content from 4 factors, while NIV content from 5 factors.

Refine search results

The average time complexity of probabilistic algorithms for finding generators in finite cyclic groups

Security of Nyberg-Rueppel digital signatures without message recovery

Barley haploid from reciprocal crosses of Hordeum bulbosum (2x) and H.Vulgare (2x)

The influence of genotypes on production of barley haploids with Hordeum bulbosum cytoplasm

The use of laser light for obtaining barley haploids by Hordeum bulbosum method

A simple test for detection of linkage

Theoretical bases for detection of linkege of genes between two quantitative characters in the presence of nonallelic interaction

Methods to predict transgressive segregation in barley and other self-pollinated crops

Numerical method for detection of linkage between genes for two metrical traits

Frequency of transgression and gene distribution in barley doubled haploid populations from first and second cycle hybrids

Genetic diversity among cultivars of spring barley revealed by random amplified polymorphic DNA (RAPD)

Assessment of RAPD markers for barley doubled haploid lines resistant and susceptible to Fusarium culmorum at seedling and adult plant growth stages

Yield reduction and mycotoxin accumulation in barley doubled haploids inoculated with Fusarium culmorum (W.G.Sm.) Sacc.

Interaction of gene effects with environments for malting quality of barley doubled haploids

Genetic determination of variability of barley doubled haploids inoculated with Fusarium culmorum (W.G.Sm.) Sacc. with regard to mycotoxin accumulation and reduction in yield traits