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1

Present-day and future state of art of reconstruction of the facial skeleton

100%
Zapala J., Wyszynska-Pawelec G.
Biotechnologia
|
2006
|
issue 1
110-124
EN
In this paper, present-day techniques used in reconstructive surgery of the facial skeleton are presented, including indications for autogenous, free and vascularized bone grafts as well as homogenous grafts, implants and biomaterials. The main directions of the development of reconstructive surgery are discussed. The preliminary results of research on xenogenous bone grafts harvested from transgenic pigs are presented as well as experimental model for New Zealand white rabbit and transgenic pig.
2

Comparison of proliferation and motile activity between human keratinocytes isolated from skin and oral muco

61%
Drukala J., Zarzecka J., Gojniczek K., Waligorska A., Zapala J., Korohoda W.
Folia Biologica
|
2005
|
vol. 53
|
issue 1-2
21-28
EN
Objective: Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. For healing of skin wounds the skin keratinocytes can be replaced by oral mucosa epithelial cells grown in vitro. The presented experiments were carried out in order to compare the proliferation, morphology, and migration between human keratinocytes isolated from human skin and oral mucosa. Materials and methods: Human epidermal and oral mucosa keratinocytes from primary culture were used in all experiments. Cell motility and shape were determined using computer-aided methods. Results and conclusions: It was demonstrated that although both cell types exhibit the same typical epithelial morphology, oral mucosa keratinocytes locomote significantly faster than skin keratinocytes. They also differ in proliferation activity. Oral mucosa keratinocytes exhibited faster growth and different actin cytoskeleton organisation than skin keratinocytes under in vitro conditions. Autologous oral mucosa keratinocytes may be expanded in vitro and used for skin wound healing in vivo.
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