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A novel repetitive DNA sequence in lemon [Citrus limon (L.) Burm.] and related species

100%

De_ F. B., Wilson R. R., Ciarmiello L., Conicella C.

issue 3

315-320

Repetitive DNA sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of such sequences provide an insight into the organization of the genome of interest. Here, we report the isolation and molecular characterization of a novel repetitive DNA sequence from the genome of Citrus limon. Digestion of C. limon DNA with MboI produced a prominent fragment of approximately 600 bp. Southern blotting revealed a ladder composed of DNA fragments that are multimers of the 600 bp Mbo I band. This suggests that MboI isolated a novel satellite, named C. limon satellite DNA 600 (CL600). Methylation analyses using Sau3AI-MboI isoschizomers demonstrated that most cytosine residues in the GATC sites of this element were methylated in C. limon. This sequence was also found in related citrus plants, like grapefruit and orange, and the hardiest close relatives of Citrus, such as kumquat and trifoliate orange.

Screening for mutations affecting sexual reproduction after activation tagging in Arabidopsis thaliana

76%

Perrella G., Cremona G., Consiglio F., Errico A., Bressan R. A., Conicella C.

Journal of Applied Genetics

2006

vol. 47

issue 2

109-111

In this work, a seed-set-based screening was performed on 70 lines of Arabidopsis thaliana after activation tagging mutagenesis to identify mutations in reproductive mechanisms. Five mutants showed significantly lower seed set than the wild type and confirmed the phenotype in the progeny. This phenotype was linked with the marker gene bar carried by T-DNA conferring glufosinate resistance. Genetic analysis revealed that the mutation inheritance was sporophytic in 3 mutants and gametophytic in 2 mutants. In addition, 2 mutants had an extra T-DNA copy. Thus activation tagging can be an effective strategy to identify new mutations affecting sporogenesis or gametogenesis.

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2 Journal of Applied Genetics

1 2006

1 2004

2 Conicella C.

1 Bressan R. A.

1 Ciarmiello L.

1 Consiglio F.

1 Cremona G.

1 De_ F. B.

1 Errico A.

1 Perrella G.

1 Wilson R. R.

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A novel repetitive DNA sequence in lemon [Citrus limon (L.) Burm.] and related species

Screening for mutations affecting sexual reproduction after activation tagging in Arabidopsis thaliana