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1

Molecular markers, their genetic background and some applications in plant genetics

100%
Bednarek P. T., Chwedorzewska K.
Biotechnologia
|
2001
|
issue 1
9-34
EN
The presented paper gives an insight into the genetic background of molecular diversity at the DNA level. Its potential sources, classified into two main categories depending on whether they lead to DNA sequence alternations (point mutations, insertions, deletions, chromosomal rearrangements, mobile elements etc) or changes in DNA methylation pattern, are discussed in parallel to their ?molecular markers? possible occurrence in the genome. A general overview of the most important sequences responsible for genetic variability (micro-, mini-, midi- and macrosatellites; transposones and retrotransposones) is given. Special attention is paid to different types of molecular marker systems and their most important applications. Molecular techniques are divided into several groups depending on the enzymes they use (endonucleases, T4 DNA ligase, Taq DNA polymerase or their combinations). Finally, investigation carried out with molecular markers in somaclonal embriogenesis is discussed.
2

Saturating rye genetic map with amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers

81%
Bednarek P. T., Masojc P., Lewandowska R., Myskow B.
Journal of Applied Genetics
|
2003
|
vol. 44
|
issue 1
21-33
EN
A linkage map of rye, previously developed using DS2 ? RXL10 F2 mapping population, was enriched with 179 AFLP and 19 RAPD marker loci. The current map covers 1386 cM and contains 480 markers including 200 RFLPs, 179 AFLPs, 88 RAPDs, 12 protein loci and one dwarfing gene. AFLPs generated by EcoRI/MseI primer combinations were distributed over the entire genome as distinct loci or clusters of 2-14 tightly linked DNA fragments. New marker loci mapped distally to the existing framework, significantly increased coverage of chromosomes 1R, 2R and 5R. The average marker distance is now 2.9 cM, but in seven regions the closest markers are still more than 20 cM apart. A detailed description of the newly mapped AFLP and RAPD loci is presented. The relationship with other published rye maps is discussed.
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