Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 4

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1
Publication available in full text mode
Content available

Optimization of activated sludge storage before RNA isolation

100%
Cydzik-Kwiatkowska A., Wnuk M.
BioTechnologia
|
2011
|
issue 1
2

Optimization of activated sludge storage before RNA isolation

100%
Cydzik-Kwiatkowska A., Wnuk M.
Biotechnologia
|
2011
|
issue 1
101-105
EN
Proper sample storage before RNA isolation is crucial for a reliable assessment of microbial activity in activated sludge. In our studies, different amounts of activated sludge expressed in mg of total suspended solids (TSS) were fixed with a constant volume of RNA later RNA Stabilization Reagent (QIAGEN, Germantown, MD, USA) and stored for 3 or 6 months. The results obtained point out that for the best RNA recovery, 1 mg of activated sludge TSS should be fixed with 33 ?l of the RNAlater. It was also observed that a longer storage period (6 vs. 3 months) of the activated sludge in RNA later lowered RNA recovery by, on average, 25 ? 15% although bacterial diversity in all samples remained unaltered.
3

Factors responsible for modulation of ribosomal RNA transcription

81%
Wnuk M., Slota E., Kotylak Z., Bugno M.
Folia Biologica
|
2006
|
vol. 54
|
issue 1-2
1-8
EN
The aetiology of variation in transcription of ribosomal genes is still an open question. Investigations carried out in many organisms showed that the variation depends not only on genetic mechanisms such as rDNA methylation, elimination of rDNA or the position effects. In this review, we discuss the results of the studies on repression of rRNA transcriptional activation from protein factors to conditions of cell culture, which may influence the variation of nucleolar organizer region activity.
4

Additional chromosome in a child as a result of a balanced reciprocal translocation t(12;18)(p13;q12) in his mother's karyotype

61%
Lassota M., Przelozna B., Plodzien M., Bugno M., Wnuk M., Kotylak Z., Slota E.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 4
419-421
EN
In this case report we present a child with an additional chromosome in the karyotype. The karyotypes of the boy and his parents were analyzed by use of a conventional banding technique (GTG) and fluorescence in situ hybridization (FISH). Probes painting whole chromosomes 12 and 18 were used in FISH. Cytogenetic examination of the parents revealed that his mother was carrying balanced reciprocal translocation between chromosomes 12 and 18. Her karyotype was described as 46,XX,t(12;18)(p13;q12). Father's karyotype was normal, described as 46,XY. The boy's karyotype was defined as 47,XY,+der(18)t(12;18)(p13;q12). The additional chromosome appeared probably due to 3:1 meiotic disjunction of the maternal balanced translocation, known as tertiary trisomy. The mother displayed a normal phenotype and delivered earlier a healthy child. However, the boy with the unbalanced karyotype shows multiple congenital abnormalities.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.