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The aim of this study was to develop a green synthesis of silver nanoparticles using mint plant leaves extracts. The fresh suspension of plant extracts was yellowish-green in color. However, after the addition of AgNO3 within 15min, the suspension showed a change in color to dark brown after 5 hours of incubation at room temperature. The formation of silver nanoparticles was confirmed using UV-Vis spectral analysis and revealed silver surface plasmon resonance band to be in a range around 200-500 nm. The XRD pattern showed the characteristic bragg peaks of (111), (200) and (220) planes of the face center cubic (FCC) silver nanoparticles AgNPS. Under scanning electron microscope (SEM), silver nanoparticles with a mean particle size of 26 nm and a spherical shape were most frequently observed. In testing, the synthesized AgNPs colloidal solution showed better antibacterial activity against both Gram-positive and Gram-negative bacterial strains. The diameter of the inhibition zones of AgNPs at 50 µg/ml concentration against bacterial strains such as Bacillus subtilis and Escherichia coli were 25 mm and 20 mm, respectively.
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