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1
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Eigenproblems in nanomechanics

100%
Muc A., Banaś A.
|
2015
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issue 3
2
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X-Ray Absorption Near Edge Structure and Mössbauer Spectroscopy in Study of Iron Valence States in Tissues

81%
Dziedzic-Kocurek K., Banaś A., Kwiatek W., Stanek J.
Acta Physica Polonica A
|
2006
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vol. 109
|
issue 3
341-345
EN
X-ray absorption near edge structure Fe K-edge spectra and ^{57}Fe Mössbauer spectra of selected standard compounds were recorded at room temperature. Valence and spin states of Fe in these samples known from Mössbauer spectroscopy were correlated with the shapes of X-ray absorption near edge structure spectra in search of possible application of X-ray absorption near edge structure spectroscopy as analytical tool determining local electronic states of iron in tissues. As an example, the X-ray absorption near edge structure spectra of healthy and cancerous tissues of prostate are shown, suggesting Fe^{3+} in cancerous tissues.
3
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Elemental Mapping of Prostate Tissue by Micro-SRIXE

71%
Banaś A., Banaś K., Falkenberg G., Dyduch G., Kwiatek W.
Acta Physica Polonica A
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2006
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vol. 109
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issue 3
323-328
EN
Synchrotron radiation-induced X-ray emission is now a proven analytical method for the determination of trace elements. Better insight of the role of trace elements in tissue samples can be gained by acquiring complete distribution maps of these elements. Our approach is based on the acquisition of digital maps. In this contribution we present two different methods for obtaining normalization matrix: first - based on argon intensity in the single spectrum and second - based on changes of beam current intensity. The region for the scan was carefully chosen according to the histological view of the sample. A total area was scanned with a step size of 15 μm in each direction and measuring time of 30 s per pixel. The thickness of the samples was 15 μm. A polycapillary was used for beam focusing. At the energy of 18 keV the beam size on the sample was approximately 15 μm which is of the order of a cell diameter.
4
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Preliminary Study on Chemical Speciation of Sulphur in Cancerous Tissues

61%
Kwiatek W., Banaś A., Banaś K., Kisiel A., Cinque G., Falkenberg G.
Acta Physica Polonica A
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2006
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vol. 109
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issue 3
383-387
EN
Sulphur is vital for almost all living organisms by participating in a wide variety of metabolic processes. Nevertheless its biochemistry is only partially understood, due to it a few tools such as X-ray absorption near edge structure may be used to determine its chemical speciation in biological system. In this paper we focused on analysis of composition and elemental distribution in tissue structures of biological samples. We examined cancerous and non-cancerous prostate tissues. Experiments were carried out on the L-beam line at the HASYLAB, DESY (Hamburg, Germany) and DAΦNE-Light Facility at the Laboratori Nationali di Frascati (Frascati, Italy). Synchrotron radiation induced X-ray emission measurements reveal the significant differences in concentration of sulphur between cancerous and non-cancerous tissues. X-ray absorption near edge structure spectra show two main types of sulphur that are represented by X-ray peaks at 2476 eV and 2484 eV. This suggests that different sulphur components are present in the measured samples.
5
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Distinguishing Prostate Cancer from Hyperplasia

52%
Kwiatek W., Banaś A., Banaś K., Podgórczyk M., Dyduch G., Falkenberg G., Gajda M., Cichocki T.
Acta Physica Polonica A
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2006
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vol. 109
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issue 3
377-381
EN
The prostate gland is the most common site of neoplastic disorders in men. The knowledge of the pathogenesis of benign prostate hyperplasia and prostate cancer is still under investigation. X-ray fluorescence analysis using synchrotron radiation seems to be an appropriate technique to study the elemental composition of biological tissues. The measurements presented in this paper were carried out on the L-beam line at the HASYLAB, DESY (Germany). The observed concentrations of Fe, Cu, Mn, and Zn are significantly higher in cancerous tissues, as compared to normal and hyperplastic ones. Our results reveal also the existence of the two types of hyperplasia.
6
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Application of μ-FTIR-SR Spectroscopy to Prostate Tissue Analysis

52%
Kwiatek W., Paluszkiewicz C., Banaś A., Kisiel A., Podgórczyk M., Marcelli A., Cestelli Guidi M., Piccinini M.
Acta Physica Polonica A
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2009
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vol. 115
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issue 2
602-605
EN
The infrared spectromicroscopy is a quite recent technique rapidly developing thanks to the availability of new instruments and new brilliant synchrotron radiation sources in different areas and in particular to biomedical researches. In order to achieve a diffraction limited spatial resolution in tissue samples, we performed experiments at SINBAD, the synchrotron infrared beamline of the Laboratori Nazionali di Frascati. We characterized the chemical composition of prostate tissue samples taken from patients affected by prostate cancer disease. Different sizes of the pinholes were considered for the measurements. In the case of prostate tissue sections the results show the possibility to determine the intensity ratio of the CH_2 and CH_3 bands set at 2930 cm^{-1} and 2960 cm^{-1}, respectively. Experiments were also performed with a pinhole of 5 μm of diameter and the differences in both histological and chemical compositions of such samples were determined.
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