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Ability of Lactobacillus plantarum LS/07 to modify intestinal enzymes activity in chronic diseases prevention

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Hijová E., Kuzma J., Strojný L., Bomba A., Bertková I., Chmelárová A., Hertelyová Z., Benetinová V., Štofilová J., Ambro Ľ.

2017

vol. 64

issue 1

113-116

The ability of probiotic strain Lactobacillus plantarum LS/07 to modify the activity of intestinal bacterial enzymes - β-glucuronidase (β-GLUCUR), β-galactosidase (β-GAL), and β-glucosidase (β-GLU) in prevention of chronic diseases - cancer, atherosclerosis and dysbiosis was investigated. The male Sprague-Dawley rats were randomly divided into 12 experimental groups: controls groups - C (control), AT (atherosclerotic), CC (carcinogenic), dysbiotic groups - each group in combination with antibiotics (ATB), probiotics groups - in combinatioan with probiotic (PRO) alone, and each group with combination of antibiotic and probiotic (ATB+PRO). In the control group the β-glucuronidase activity did not change throughout the experiment. High fat diet in atherosclerotic group significantly increased the activity of β-glucuronidase (P<0.001) and β-glucosidase (P<0.01). Azoxymethane application in carcinogenic group significantly increased β-glucuronidase (P<0.01), but reduced β-glucosidase (P<0.01) activity. Daily application of probiotics alone and in combination with antibiotic increased β-galactosidase, of β-glucosidase, and decreased β-glucuronidase activity. In control antibiotic group we observed significant increase in β-glucuronidase (P<0.05) and decreased β-glucosidase (P<0.01) activity which can be caused by the change of microflora in favor of coliform bacteria. These findings indicate the positive effects of probiotic Lactobacillus plantarum LS/07 and suggest its use in disease prevention in human medicine and some animal species.

Markers of oxidative stress in acute myocardial infarction treated by percutaneous coronary intervention

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Sedláková E., Rácz O., Lovásová E., Beòaèka R., Kurpas M., Chmelárová A., Sedlák J., Studenèan M.

Open Medicine

2009

vol. 4

issue 1

26-31

In the current study, we evaluated the dynamics of oxidative stress markers in patients with acute myocardial infarction (AMI) treated by primary percutaneous coronary intervention (PCI). Thirty consecutive patients with AMI with ST elevation were included. Plasma lipid peroxidation end product malondialdehyde (MDA) and total antioxidant capacity (TAC) in blood plasma were evaluated. Peripheral venous blood samples were obtained prior to reperfusion and at five time points after reperfusion. The control group consisted of 20 ischemic patients without acute coronary syndrome. TAC in the AMI group at admission was lower than in control patients (1.26 + 0.32 vs. 1.52 + 0.24 mmol/l). Within 1 h after reperfusion, in most cases, values significantly declined (1 min, 1.10 + 0.33 mmol/l; 1 h, 1.06 + 0.21 mmol/l [p= 0.03]). After 3 h, values began to increase (1.14 + 0.29 mmol/l) and returned to basal values after 3 d (1.29 + 0.24 mmol/l). MDA levels in AMI patients at admission were higher than in control patients (1.66 + 0.55 vs. 1.44 + 0.55 mmol/l) but showed a sustained decrease over the 3 h after reperfusion of the occluded artery (1 min, 1.57 + 0.37 mmol/l; 1 h, 1.50 + 0.35 μmol/l; 3 h, 1.35 + 0.59 μmol/l [p = 0.03]). Reperfusion of the occluded coronary artery by PCI in AMI lead to an immediate decrease in TAC, suggesting formation of reactive oxygen species. However, the MDA level significantly decreased after reperfusion. This may suggests less reperfusion injury after PCI.

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1 Acta Biochimica Polonica

1 Open Medicine

1 2017

1 2009

2 Chmelárová A.

1 Ambro Ľ.

1 Benetinová V.

1 Bertková I.

1 Beòaèka R.

1 Bomba A.

1 Hertelyová Z.

1 Hijová E.

1 Kurpas M.

1 Kuzma J.

1 Lovásová E.

1 Rácz O.

1 Sedlák J.

1 Sedláková E.

1 Strojný L.

1 Studenèan M.

1 Štofilová J.

Search results

Ability of Lactobacillus plantarum LS/07 to modify intestinal enzymes activity in chronic diseases prevention

Markers of oxidative stress in acute myocardial infarction treated by percutaneous coronary intervention