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Analysis of gypsogenin saponins in homeopathic tinctures

100%
Szakiel A., Henry M.
|
2007
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vol. 54
|
issue 4
853-856
EN
A relatively simple and short procedure for the quantitative determination of gypsogenin saponins was performed to evaluate homeopathic tinctures in which those compounds can be regarded as one of the active constituents. This method comprises partial hydrolysis of saponins, subsequent extraction of liberated prosaponin (gypsogenin 3-O-glucuronide) and its analysis by high performance liquid chromatography. Glycyrrhizic acid was used as an internal standard. This method was successfully applied to the analysis of mother tinctures obtained from Saponaria officinalis. Thus, the determination of triterpenoid saponins can be used as a convenient and sufficient method of standardization of selected homeopathic tinctures.
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New method for the study of Amaryllidaceae alkaloid biosynthesis using biotransformation of deuterium-labeled precursor in tissue cultures

52%
El Tahchy A., Boisbrun M., Ptak A., Dupire F., Chrétien F., Henry M., Chapleur Y., Laurain-Mattar D.
|
2010
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vol. 57
|
issue 1
75-82
EN
Biotransformation of deuterated-4'-O-methylnorbelladine into alkaloids galanthamine and lycorine in tissue cultures of Leucojum aestivum was demonstrated using HPLC coupled to mass spectrometry. GC-MS screening was also carried to investigate other native and deuterated alkaloids. A total of six labeled alkaloids were identified indicating that 4'-O-methyl-d3-norbelladine is incorporated into three different groups of Amaryllidaceae alkaloids that are biosynthesized by three modes of intramolecular oxidative phenol coupling.
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