Search results

Sort By:

Limit search:

Energy Dissipation in the AFM Elasticity Measurements

100%

Klymenko O., Wiltowska-Zuber J., Lekka M., Kwiatek W.

Acta Physica Polonica A

2009

vol. 115

issue 2

548-551

Nowadays, it is well established that changes of cell stiffness observed by atomic force microscopy are linked with the cell cytoskeleton. Its structural and functional alterations are underlying major diseases such as cancer, inflammation or neurodegenerative disorders. So far, the use of atomic force microscopy is mostly focused on the determination of the Young modulus using the modified Hertz model. It can quantitatively describe the elastic properties of living cells, however, its value is burdened by the fact that cells are neither isotropic nor homogeneous material. Often, during the atomic force microscopy measurements, the hysteresis between the loading and unloading curves are observed which indicates the dissipation of an energy. In our studies, the index of plasticity was introduced to enumerate such effect during a single loading-unloading cycle. As the results show, such approach delivers an additional parameter describing the mechanical state of cell cytoskeleton. The analysis was performed on test samples where the mechanical properties of the melanoma cells were changed by glutaraldehyde and cytochalasin D treatments. The non-treated cells were compared with fibroblasts.

Refine search results

1 Acta Physica Polonica A

1 2009

1 Klymenko O.

1 Kwiatek W.

1 Lekka M.

1 Wiltowska-Zuber J.

Search results

Energy Dissipation in the AFM Elasticity Measurements